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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Combined transcription factor profiling, microarray analysis and metabolite profiling reveals the transcriptional control of metabolic shifts occurring during tomato fruit development
Plant Journal, Volume 68, No. 6, Year 2011
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Description
Maturation of fleshy fruits such as tomato (Solanum lycopersicum) is subject to tight genetic control. Here we describe the development of a quantitative real-time PCR platform that allows accurate quantification of the expression level of approximately 1000 tomato transcription factors. In addition to utilizing this novel approach, we performed cDNA microarray analysis and metabolite profiling of primary and secondary metabolites using GC-MS and LC-MS, respectively. We applied these platforms to pericarp material harvested throughout fruit development, studying both wild-type Solanum lycopersicum cv. Ailsa Craig and the hp1 mutant. This mutant is functionally deficient in the tomato homologue of the negative regulator of the light signal transduction gene DDB1 from Arabidopsis, and is furthermore characterized by dramatically increased pigment and phenolic contents. We choose this particular mutant as it had previously been shown to have dramatic alterations in the content of several important fruit metabolites but relatively little impact on other ripening phenotypes. The combined dataset was mined in order to identify metabolites that were under the control of these transcription factors, and, where possible, the respective transcriptional regulation underlying this control. The results are discussed in terms of both programmed fruit ripening and development and the transcriptional and metabolic shifts that occur in parallel during these processes. © 2011 Blackwell Publishing Ltd.
Authors & Co-Authors
Rohrmann, Johannes
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Tohge, Takayuki
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Alba, Rob
United States, Ithaca
Usda Ars Robert W. Holley Center for Agriculture and Health
United States, St. Louis
Monsanto Company
Osorio, Sonia
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Caldana, Camila
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
McQuinn, Ryan P.
United States, Ithaca
Usda Ars Robert W. Holley Center for Agriculture and Health
Arvidsson, Samuel
Germany, Potsdam
Universität Potsdam
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
van der Merwe, Margaretha J.
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
South Africa, Stellenbosch
Stellenbosch University
Riaño-Pachón, Diego Mauricio
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Colombia, Bogota
Universidad de Los Andes, Colombia
Mueller-Roeber, Bernd
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Germany, Potsdam
Universität Potsdam
Fei, Zhangjun Jun
United States, Ithaca
Usda Ars Robert W. Holley Center for Agriculture and Health
Nunes-Nesi, Adriano
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Brazil, Vicosa
Universidade Federal de Vicosa
Giovannoni, James J.
United States, Ithaca
Usda Ars Robert W. Holley Center for Agriculture and Health
Fernie, Alisdair Robert
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Statistics
Citations: 115
Authors: 14
Affiliations: 7
Identifiers
Doi:
10.1111/j.1365-313X.2011.04750.x
ISSN:
09607412
e-ISSN:
1365313X
Research Areas
Genetics And Genomics
Noncommunicable Diseases
Study Approach
Quantitative