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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Methods for detection of Trichomonas vaginalis in the male partners of infected women: Implications for control of trichomoniasis
Journal of Clinical Microbiology, Volume 44, No. 11, Year 2006
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Description
Trichomonas vaginalis infection in men is an important cause of nongonococcal urethritis. Effective detection of the parasite in men using culture requires examination of multiple specimens. We compared culture and PCR-enzyme-linked immunosorbent assay in urethral swabs, urine, and semen for T. vaginalis detection in male sexual partners of women with trichomoniasis identified by wet mount and culture. Trichomonads were detected by at least one positive test in 205/280 men (73.2%) who submitted at least one specimen for culture and PCR. Whereas InPouch TV culture detected only 46/205 cases (22.5%), PCR detected 201/205 (98.0%). Urethral swab cultures from men with urethritis were more likely to be positive with shorter incubation than specimens from men without urethritis. T. vaginalis was detected more often in men with wet-mount-positive partners. Even with a sensitive PCR assay, reliable detection of T. vaginalis in male partners required multiple specimens. The majority of male sexual partners in this study were infected, emphasizing the importance of partner evaluation and treatment. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Hobbs, Marcia M.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Lapple, Dana M.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Schwebke, Jane R.
United States, Birmingham
The University of Alabama at Birmingham
Cohen, Myron S.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Atashili, Julius
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Leone, Peter A.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Miller, William C.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Seña, Arlene Carmela
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Statistics
Citations: 72
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1128/JCM.00952-06
ISSN:
00951137
Research Areas
Sexual And Reproductive Health
Participants Gender
Male
Female