Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda
Journal of General Virology, Volume 90, No. 11, Year 2009
Notification
URL copied to clipboard!
Description
The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest the existence of a large number of undiscovered flaviviruses. Recently, the first isolation of cell fusing agent virus (CFAV) was reported from a natural mosquito population in Puerto Rico, and sequences related to CFAV have been discovered in mosquitoes from Thailand. CFAV had previously been isolated from a mosquito cell line in 1975 and represented the only known 'insect-only' flavivirus, appearing to replicate in insect cells alone. A second member of the 'insect-only' group, Kamiti River virus (KRV), was isolated from Kenyan mosquitoes in 2003. A third tentative member of the 'insect-only' group, Culex flavivirus (CxFV), was first isolated in 2007 from Japan and further strains have subsequently been reported from the Americas. We report the discovery, isolation and characterization of two novel 'insect-only' flaviviruses from Entebbe, Uganda: a novel lineage tentatively designated Nakiwogo virus (NAKV) and a new strain of CxFV. The individual mosquitoes from which these strains were isolated, identified retrospectively by using a reference molecular phylogeny generated using voucher specimens from the region, were Mansonia africana nigerrima and Culex quinquefasciatus, respectively. This represents the first isolation, to our knowledge, of a novel insect-only flavivirus from a Mansonia species and the first isolation of a strain of CxFV from Africa. © 2009 SGM.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2885038/bin/supp_90_11_2669__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC2885038/bin/supp_90_11_2669__1.pdf
Authors & Co-Authors
Cook, Shelley
Unknown Affiliation
Moureau, Grégory N.
Unknown Affiliation
Harbach, Ralph E.
Unknown Affiliation
Mukwaya, Louis G.
Unknown Affiliation
Goodger, Kim
Unknown Affiliation
Ssenfuka, Fred
Unknown Affiliation
Gould, Ernest Andrew
Unknown Affiliation
Holmes, Edward C.
Unknown Affiliation
De-Lamballerie, Xavier Nicolas
Unknown Affiliation
Statistics
Citations: 136
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1099/vir.0.014183-0
ISSN:
00221317
e-ISSN:
14652099
Study Design
Cross Sectional Study
Study Locations
Uganda