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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Expression of GB virus C NS5A protein from genotypes 1, 2, 3 and 5 and a 30 aa NS5A fragment inhibit human immunodeficiency virus type 1 replication in a CD4
+
T-lymphocyte cell line
Journal of General Virology, Volume 88, No. 12, Year 2007
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Description
GB virus type C (GBV-C) is a common human flavivirus that has been associated with prolonged survival in HIV-positive individuals in several, though not all, epidemiological studies. There are five distinct GBV-C genotypes that are geographically localized, and it has been speculated that GBV-C genotypic differences may explain variable outcomes observed in different clinical studies. Expression of an 85 aa fragment of the GBV-C NS5A phosphoprotein (genotype 2) in a CD4+ T cell line (Jurkat) resulted in inhibition of HIV replication, mediated in part by decreased surface expression of the HIV coreceptor CXCR4 and upregulation of SDF-1. We expressed the NS5A protein from genotypes 1, 2, 3 and 5 in Jurkat cells, and demonstrated that all genotypes inhibited HIV replication. Further deletion mapping demonstrated that expression of a 30 aa fragment resulted in decreased CXCR4 surface expression, upregulation of SDF-1 and inhibition of HIV replication. © 2007 SGM.
Authors & Co-Authors
Chang, Qing
United States, Iowa City
University of Iowa
McLinden, James H.
United States, Iowa City
University of Iowa
Stapleton, Jack T.
United States, Iowa City
University of Iowa
Sathar, Mahomed Aslam
South Africa, Durban
The Nelson R. Mandela Medical School
Xiang, Jinhua
United States, Iowa City
University of Iowa
Statistics
Citations: 30
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1099/vir.0.83198-0
ISSN:
00221317
Research Areas
Genetics And Genomics
Infectious Diseases