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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Antileukemia component, dehydroeburicoic acid from Antrodia camphorata induces DNA damage and apoptosis in vitro and in vivo models
Phytomedicine, Volume 19, No. 8-9, Year 2012
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Description
Antrodia camphorata (AC) is a native Taiwanese mushroom which is used in Asian folk medicine as a chemopreventive agent. The triterpenoid-rich fraction (FEA) was obtained from the ethanolic extract of AC and characterized by high performance liquid chromatography (HPLC). FEA caused DNA damage in leukemia HL 60 cells which was characterized by phosphorylation of H2A.X and Chk2. It also exhibited apoptotic effect which was correlated to the enhancement of PARP cleavage and to the activation of caspase 3. Five major triterpenoids, antcin K (1), antcin C (2), zhankuic acid C (3), zhankuic acid A (4), and dehydroeburicoic acid (5) were isolated from FEA. The cytotoxicity of FEA major components (1-5) was investigated showing that dehydroeburicoic acid (DeEA) was the most potent cytotoxic component. DeEA activated DNA damage and apoptosis biomarkers similar to FEA and also inhibited topoisomerase II. In HL 60 cells xenograft animal model, DeEA treatment resulted in a marked decrease of tumor weight and size without any significant decrease in mice body weights. Taken together, our results provided the first evidence that pure AC component inhibited tumor growth in vivo model backing the traditional anticancer use of AC in Asian countries. © 2012 Elsevier GmbH. All rights reserved.
Authors & Co-Authors
Du, Yingchi
Taiwan, Kaohsiung
Kaohsiung Medical University
Chang, Fangrong
Taiwan, Kaohsiung
Kaohsiung Medical University
Taiwan, Kaohsiung
Kaohsiung Medical University Chung-ho Memorial Hospital
Taiwan, Kaohsiung
National Sun Yat-sen University
Wu, Tungying
Taiwan, Kaohsiung
Kaohsiung Medical University
Hsu, Yuming
Taiwan, Kaohsiung
Kaohsiung Medical University
El-Shazly, Mohamed
Taiwan, Kaohsiung
Kaohsiung Medical University
Egypt, Cairo
Faculty of Pharmacy - Ain Shams University
Chen, Chieh Fu
Taiwan, Taipei
Chih-ying Plant Research and Development Foundation
Sung, Ping Jyun Sung
Taiwan, Shoufeng
National Dong Hwa University
Taiwan, Checheng
National Museum of Marine Biology and Aquarium Taiwan
Lin, Yan Yu
Taiwan, Kaohsiung
Kaohsiung Medical University
Lin, Yi Hsin
Taiwan, Kaohsiung
Kaohsiung Medical University
Wu, Yangchang
Taiwan, Kaohsiung
Kaohsiung Medical University
Taiwan, Taichung
China Medical University
Taiwan, Taichung
China Medical University Hospital
Lu, Mei Chin
Taiwan, Kaohsiung
Kaohsiung Medical University
Taiwan, Shoufeng
National Dong Hwa University
Taiwan, Checheng
National Museum of Marine Biology and Aquarium Taiwan
Statistics
Citations: 39
Authors: 11
Affiliations: 9
Identifiers
Doi:
10.1016/j.phymed.2012.03.014
ISSN:
09447113
e-ISSN:
1618095X
Research Areas
Cancer
Genetics And Genomics
Health System And Policy