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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
A multiplex PCR to differentiate the two sibling species of mosquitoes Ochlerotatus detritus and Oc. coluzzii and evidence for further genetic heterogeneity within the Detritus complex
Infection, Genetics and Evolution, Volume 28, Year 2014
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Description
Internal transcribed spacer regions of ribosomal DNA were sequenced, and species-specific primers were designed to simplify the identification of two morphologically similar species of the Detritus complex, Ochlerotatus detritus and Ochlerotatus coluzzii. Each newly designed primer was able to amplify a species-specific fragment with a different size. Samples from France and Germany were successfully tested. This new tool prompts for bio-ecological studies to refine basic knowledge on the bionomics of this species complex, towards a better control and prevention of ensuing nuisances. Moreover, ITS2 sequencing revealed the existence of (1) two distinct haplotypes of Oc. detritus that are sympatric and widely distributed along the French Atlantic and Mediterranean littorals and (2) a specific haplotype in mosquitoes sampled from Tunisia, raising the question of the taxonomic status of this North-African population. © 2014 Elsevier B.V.
Authors & Co-Authors
Brengues, Cécile
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Ferré, Jean Baptiste
France, Montpellier
Entente Interdépartementale Pour la Démoustication du Littoral Méditerranéen
Le Goff, Gilbert
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Lami, Patrick
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Pratlong, Francine
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Pasteur, Nicole
France, Montpellier
Université de Montpellier
Lagneau, Christophe
France, Montpellier
Entente Interdépartementale Pour la Démoustication du Littoral Méditerranéen
Simard, Frédéric R.
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Robert, Vincent
France, Montpellier
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Statistics
Citations: 11
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1016/j.meegid.2014.07.010
ISSN:
15671348
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Tunisia