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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Expression of viral EPS-depolymerase reduces fire blight susceptibility in transgenic pear
Plant Cell Reports, Volume 23, No. 9, Year 2005
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Description
Erwinia amylovora is the causal agent of fire blight of Maloideae. One of the main pathogenicity factors of this bacterium is the exopolysaccharide (EPS) of its capsule. In this paper, we used genetic transformation tools to constitutively express an EPS-depolymerase transgene in the pear (Pyrus communis L.) cv. Passe Crassane with the aim of decreasing its high susceptibility to fire blight. Expression of the depolymerase gene in 15 independent transgenic clones led, on average, to low depolymerase activity, although relatively high expression was observed at the transcriptional and translational levels. Only two of the transgenic clones (9X and 10M) consistently showed a decrease in fire blight susceptibility in vitro and in the greenhouse. These clones were also among the highest expressers of depolymerase at the RNA and enzyme activity levels. The correlation observed among all transgenic clones between depolymerase expression and fire blight resistance suggested the potential of this strategy. © Springer-Verlag 2004.
Authors & Co-Authors
Malnoy, Mickael
France, Beaucouze
Umr Genhort
United States, Ithaca
Cornell University
Faize, Mohamed
France, Beaucouze
Umr Genhort
Japan, Tsukuba
Institute for Agro-environmental Sciences, Naro
Venisse, Jean Stéphane
France, Beaucouze
Umr Genhort
France, Clermont-ferrand
Génétique Diversité et Ecophysiologie Des Céréales Gdec
Geider, Klaus
Germany, Quedlinburg
Julius Kühn-institut - Federal Research Centre for Cultivated Plants
Statistics
Citations: 31
Authors: 4
Affiliations: 5
Identifiers
Doi:
10.1007/s00299-004-0855-2
ISSN:
07217714
Research Areas
Genetics And Genomics