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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
A simple method for the preparation of sperm of external fertilizers for scanning electron microscopy
Biotechnic and Histochemistry, Volume 53, No. 4, Year 1978
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Description
A simple technique is described for the study of sperm of some external fertilizers (sea urchin, Parechinus angulosus; teleost, Liza dumerili; anurans, Xenopus laevis and Bufo rangeri) by means of scanning electron microscopy. The technique involves: (i) dilution of sperm with water of the natural environment of the animals or artificial media resembling their natural environment; (ii) spreading of sperm suspension on 10-mm square Perspex plates; (iii) fixation of diluted sperm with 2.5 pH 7.4 Millonig phosphate buffered glutaraldehyde (1 hour); (iv) repeated washing in phosphate buffer, air drying and coating with palladium-gold in a vacuum evaporator. Postfixation with 1% osmium tetroxide did not improve the results and dehydration with an alcohol series was not necessary. © 1978 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Authors & Co-Authors
van der Horst, Gerhard
South Africa, Gqeberha
Nelson Mandela University
Cross, Robin H.M.
South Africa, Grahamstown
University of Rhodes
Statistics
Citations: 4
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.3109/10520297809111464
ISSN:
10520295
Research Areas
Environmental
Substance Abuse