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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
neuroscience
Profiling metabolite changes in the neuronal differentiation of human striatal neural stem cells using
1
H-magnetic resonance spectroscopy
NeuroReport, Volume 24, No. 18, Year 2013
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Description
Objective: Neural stem cells (NSCs) have been found to play an increasing clinical role in stroke. However, at present, it is not yet possible to noninvasively monitor their differentiation once implanted into the brain. Methods: Here, we describe the use of high-resolution H-magnetic resonance spectroscopy (MRS) to define a metabolite profile of undifferentiated human striatal NSCs from the STROC05 cell line and their differentiation after 3-weeks of treatment with purmorphamine. Results: The undifferentiated conditions were characterized by ~95% of cells expressing nestin and ~77% being Ki67(+)ve, indicating that these were still proliferating. Phosphophocholine+glycerophosphocholine (PC+GPC) as well as myo-Inositol (mI) were increased in these cells. PC+GPC and mI were markedly reduced upon differentiation, potentially serving as markers of the NSC state. Upon differentiation (~45% neurons, ~30% astrocytes, ~13% oligodendrocytes), the concentration of many metabolites decreased in absolute value. The decreasing trend of the N-acetyl-aspartate level was observed in differentiated cells when compared with NSCs. An increase in plasmalogen (enriched in myelin sheets) could potentially serve as a marker of oligodendrocytes. Conclusion: These metabolite characteristics of undifferentiated and differentiated NSCs provide a basis for exploration of their possible use as markers of differentiation after cell transplantation. © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Authors & Co-Authors
Chung, Yuen Li
United Kingdom, London
The Institute of Cancer Research
Akabawy, Gehan El
Egypt, Shibin el Kom
Menoufia University Faculty of Medicine
So, Po Wah
United Kingdom, London
King's College London
Solanky, Bhavana S.
United Kingdom, London
Ucl Queen Square Institute of Neurology
Leach, Martin O.
United Kingdom, London
The Institute of Cancer Research
Modo, Michel M.J.
United States, Pittsburgh
Mcgowan Institute for Regenerative Medicine
Statistics
Citations: 8
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1097/WNR.0000000000000056
ISSN:
09594965
e-ISSN:
1473558X
Research Areas
Cancer
Noncommunicable Diseases