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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan
Genetic Resources and Crop Evolution, Volume 54, No. 5, Year 2007
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Description
Accurate and reliable means for identification are necessary to assess the discrimination between landraces of tetraploid wheat [T. turgidum L. subsp. durum (Desf.) Husn.] and hexaploid wheat (T. aestivum L. em. Thell.). In Afghanistan, farmers usually cultivate mixed landraces, and thus distinction between bread and durum is difficult. A set of 18 microsatellites derived from the DuPont EST-database were used to describe genetic diversity in a sample of 82 Afghan wheat landraces. A total of 101 alleles were detected, with allele number per locus ranging from 2 to 13, and a mean allele number of 6.31. The percentage of polymorphic loci was 89%. The EST-SSRs markers showed different level of gene diversity: the highest Polymorphism Information Content value (0.921) was observed with DuPw 221. Our results demonstrated that with a reasonable number of expressed sequences target microsatellites (EST-SSRs) it is possible to discriminate between T. durum and T. aestivum species of wheat germplasm. Our results showed that EST-databases could be a useful source for species-specific markers and have the potential for new genic microsatellites markers that could enhance screening germplasm in gene banks. © 2007 Springer Science+Business Media, Inc.
Authors & Co-Authors
Chabane, K.
Lebanon, Beirut
International Center for Agricultural Research in the Dry Areas Syria
Abdalla, Osman Suliman
Syrian Arab Republic
Integrated Gene Management Program
Sayed, Haitham
Syrian Arab Republic
Integrated Gene Management Program
Valkoun, Jan
Lebanon, Beirut
International Center for Agricultural Research in the Dry Areas Syria
Statistics
Citations: 8
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1007/s10722-006-9193-2
ISSN:
09259864
Research Areas
Genetics And Genomics