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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
environmental science
DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus
Environmental Toxicology and Pharmacology, Volume 36, No. 2, Year 2013
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Description
The present study was undertaken to evaluate the genotoxic and oxidative stress modulatory effects of commercial formulation of glyphosate-based herbicide (Roundup®) in freshwater fish Channa punctatus. Three sublethal test concentrations of the herbicide viz., SL-I (1/10th of LC50=~3.25mgL-1), SL-II (1/8th of LC50=~4.07mgL-1) and SL-III (1/5th of LC50=~6.51mgL-1) were calculated using 96-LC50 value and the test specimens were exposed to these concentrations. Blood and gill cells of the exposed specimens were sampled on day 1, 7, 14, 21, 28 and 35 to examine the DNA damage using comet assay and to assess the alteration in lipid peroxidation and antioxidant enzymes activities. The highest DNA damage was observed on day 14 at all test concentrations followed by gradual non-linear decline. Induction of oxidative stress in the blood and gill cells were evidenced by increased lipid peroxidation level, while antioxidants namely superoxide dismutase, catalase and glutathione reductase responded in a concentration-dependent manner. The results supported the integrated use of comet and antioxidant assays in determining the toxicity of water pollutants which could be used as part of monitoring programs. © 2013 Elsevier B.V.
Authors & Co-Authors
Nwani, Christopher Didigwu
Nigeria, Naukka
University of Nigeria
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Nagpure, Naresh Sahebrao
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Kumar, Ravindra
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Kushwaha, Basdeo
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Lakra, Wazir Singh
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Statistics
Citations: 97
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/j.etap.2013.06.001
ISSN:
13826689
e-ISSN:
18727077
Research Areas
Environmental
Genetics And Genomics