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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Characterization of extended-spectrum β-lactamase-producing isolates of Haemophilus parainfluenzae
Journal of Antimicrobial Chemotherapy, Volume 61, No. 3, Year 2008
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Description
Objectives: To characterize the β-lactam resistance mechanisms of two clinical isolates of cefotaxime-resistant Haemophilus parainfluenzae recovered from patients in South Africa. Methods: The relatedness of isolates and plasmids was assessed using PFGE and restriction enzyme analysis, respectively. Plasmid-mediated and chromosomally integrated blaTEM genes and ftsI genes were sequenced, and the plasmid-mediated blaTEM-15 was used to transform a range of control organisms. Results: The two isolates were found to be unique according to PFGE, but had an identical 3.7 kb plasmid encoding a TEM-15 β-lactamase. Both isolates also had substitutions in penicillin binding protein 3 (PBP3) consistent with substitutions known to exist in β-lactamase-negative ampicillin-resistant (BLNAR) strains of Haemophilus influenzae. The cefotaxime MICs for control strains of H. influenzae, H. parainfluenzae and BLNAR H. influenzae transformed with the plasmid-mediated blaTEM-15 were 1.0, 1.0 and 4.0 mg/L, respectively, compared with 16.0 and 8.0 mg/L, respectively, for the two parent H. parainfluenzae. Conclusions: The high-level cefotaxime resistance in the H. parainfluenzae isolates was due to a combination of a plasmid-mediated TEM-15 extended-spectrum β-lactamase with altered PBP3 probably contributing. Other contributing resistance mechanisms could not be excluded. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Authors & Co-Authors
Tristram, Stephen G.
Australia, Hobart
University of Tasmania
Pitout, Marthinus J.
South Africa, Pretoria
University of Pretoria
Forward, Karen
Canada, Halifax
Dalhousie University
Campbell, Sarah J.
Canada, Halifax
Qeii Health Sciences Centre
Nichols, Scott
Australia, Hobart
University of Tasmania
Davidson, Ross J.
Canada, Halifax
Dalhousie University
Canada, Halifax
Qeii Health Sciences Centre
Statistics
Citations: 6
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1093/jac/dkm523
ISSN:
03057453
e-ISSN:
14602091
Research Areas
Cancer
Study Locations
South Africa