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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Proteomic analysis of human spermatozoa proteins with oxidative stress
Reproductive Biology and Endocrinology, Volume 11, No. 1, Article 48, Year 2013
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Description
Background: Oxidative stress plays a key role in the etiology of male infertility. Significant alterations in the sperm proteome are associated with poor semen quality. The aim of the present study was to examine if elevated levels of reactive oxygen species cause an alteration in the proteomic profile of spermatozoa.Methods: This prospective study consisted of 52 subjects: 32 infertile men and 20 normal donors. Seminal ejaculates were classified as ROS+ or ROS- and evaluated for their proteomic profile. Samples were pooled and subjected to LC-MS/MS analysis through in-solution digestion of proteins for peptide characterization. The expression profile of proteins present in human spermatozoa was examined using proteomic and bioinformatic analysis to elucidate the regulatory pathways of oxidative stress.Results: Of the 74 proteins identified, 10 proteins with a 2-fold difference were overexpressed and 5 were underexpressed in the ROS+ group; energy metabolism and regulation, carbohydrate metabolic processes such as gluconeogenesis and glycolysis, protein modifications and oxidative stress regulation were some of the metabolic processes affected in ROS+ group.Conclusions: We have identified proteins involved in a variety of functions associated with response and management of oxidative stress. In the present study we focused on proteins that showed a high degree of differential expression and thus, have a greater impact on the fertilizing potential of the spermatozoa. While proteomic analyses identified the potential biomarkers, further studies through Western Blot are necessary to validate the biomarker status of the proteins in pathological conditions. © 2013 Sharma et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Sharma, Rakesh Kumar
United States, Cleveland
Cleveland Clinic Foundation
Agarwal, Ashok K.
United States, Cleveland
Cleveland Clinic Foundation
Mohanty, Gayatri
United States, Cleveland
Cleveland Clinic Foundation
India, Cuttack
Ravenshaw University
Hamada, Alaa
United States, Cleveland
Cleveland Clinic Foundation
Gopalan, Banu
United States, Cleveland
Cleveland Clinic Foundation
Willard, Belinda
United States, Cleveland
Cleveland Clinic Foundation
Yadav, Satya P.
United States, Cleveland
Cleveland Clinic Foundation
Du Plessis, Stefan Stephanus
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 96
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1186/1477-7827-11-48
e-ISSN:
14777827
Research Areas
Noncommunicable Diseases
Sexual And Reproductive Health
Study Design
Cohort Study
Participants Gender
Male