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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
2B or not 2B? Disparate discrimination of functional VWF discordance using different assay panels or methodologies may lead to success or failure in the early identification of type 2B VWD
Thrombosis and Haemostasis, Volume 98, No. 2, Year 2007
Notification
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Description
Laboratory proficiency in the identification of functional von Willebrand factor (VWF) discordance in type 2B von Willebrand disease (VWD) was assessed by external quality assurance surveys conducted by the RCPA Haematology QAP, and using six different type 2BVWD plasma samples (three historical and three previously unpublished) tested by up to 52 laboratories. For the three most recent samples, functional VWF discordance was either not identified in testing or by interpretation with misidentification as 'normal' or 'type I VWD', on average for 25.7% of test occasions when laboratories performed VWF:Ag and VWF:RCo as their primary VWF test panel, but somewhat fewer occasions (10.9%) for laboratories that incorporated VWF:CB as an additional functional VWF assay. VWF assay sub-methodologies also influenced the appropriate identification of samples as potentially type 2 VWD, and VWF functional discordance was more consistently identified when laboratories used (i) automated platelet agglutination for VWF:RCo compared to classical platelet aggregometry, (ii) inhouse VWF:CB assays compared to commercial kit methods, and (iii) automated LIA-based 'VWF:Activity' assays compared to ELISA based assays. We conclude that: (i) laboratories are generally proficient in tests VWD but interpretative diagnostic errors do occur; (ii) correct diagnosis is more likely when test panels are more comprehensive and include the VWF:CB; (iii) sub-methodology influences the appropriate identification of VWF functional discordance. On the basis of these findings, we provide a series of recommendations to enable the appropriate laboratory identification of VWD, in particular type 2BVWD. © 2007 Schattauer GmbH, Stuttgart.
Authors & Co-Authors
Favaloro, Emmanuel J.
Australia, Parramatta
Institute of Clinical Pathology and Medical Research
Bonar, Roslyn
Australia, Parramatta
Institute of Clinical Pathology and Medical Research
Meiring, Muriel S.
South Africa, Bloemfontein
University of the Free State, School of Medicine
Street, Alison M.
Australia, Parramatta
Institute of Clinical Pathology and Medical Research
Marsden, Katherine
Australia, Parramatta
Institute of Clinical Pathology and Medical Research
Statistics
Citations: 46
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1160/TH06-12-0693
ISSN:
03406245
Study Design
Cross Sectional Study