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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Tetravalent dengue DNA vaccine is not immunogenic when delivered by retrograde infusion into salivary glands
Tropical Diseases, Travel Medicine and Vaccines, Volume 6, No. 1, Article 10, Year 2020
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Description
Introduction and background: A tetravalent DNA vaccine for Dengue virus is under development but has not yet achieved optimal immunogenicity. Salivary glands vaccination has been reported efficacious in rodents and dogs. We report on a pilot study testing the salivary gland as a platform for a Dengue DNA vaccine in a non-human primate model. Materials and methods: Four cynomolgus macaques were used in this study. Each macaque was pre-medicated with atropine and sedated with ketamine. Stensen's duct papilla was cannulated with a P10 polyethylene tube, linked to a 500ul syringe. On the first two infusions, all macaques were infused with 300ul of TVDV mixed with 2 mg of zinc. For the 3rd infusion, to increase transfection into salivary tissue, two animals received 100uL TVDV mixed with 400uL polyethylenimine 1μg/ml (PEI) and the other two animals received 500uL TVDV with zinc. Antibody titers were assessed 4 weeks following the second and third infusion. Results and conclusions: SGRI through Stensen's duct is a well-tolerated, simple and easy to reproduce procedure. TVDV infused into macaques salivary glands elicited a significantly weaker antibody response than with different delivery methods. © 2020 The Author(s).
Authors & Co-Authors
Caudell, David L.
United States, Winston Salem
Wake Forest University School of Medicine
Raviprakash, Kanakatte S.
United States, Silver Spring
Naval Medical Research Center
Williams, Maya
United States, Silver Spring
Naval Medical Research Center
Porter, Kevin Randall
United States, Silver Spring
Naval Medical Research Center
Sanders, John Walton
United States, Winston Salem
Wake Forest University School of Medicine
Statistics
Citations: 2
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1186/s40794-020-00111-5
ISSN:
20550936
Research Areas
Genetics And Genomics
Infectious Diseases