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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches
Journal of Zhejiang University: Science B, Volume 14, No. 12, Year 2013
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Description
Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various troublesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues (roots and leaves) of Tunisian date palm trees (Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar (PDA) medium and identified by a sequence match search wherein their 18S-28S internal transcribed spacer (ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fell into two groups, namely Nectriaceae and Pleosporaceae. Additionally, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. Restriction fragment length polymorphism (RFLP) analysis of the ITS from 200 fungal clones (leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiella (Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria (members of the Nectriaceae family), the leaves were essentially colonized by Alternaria (members of the Pleosporaceae family). Overall, the findings of the present study constitute, to the authors' knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees (P. dactylifera). © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg.
Authors & Co-Authors
Ben Chobba, Ines
Tunisia, Sfax
Faculté Des Sciences de Sfax
Elleuch, Amine
Tunisia, Sfax
Faculté Des Sciences de Sfax
Ayadi, Imen
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Khannous, Lamia
Tunisia, Sfax
Faculté Des Sciences de Sfax
Namsi, A.
Tunisia, Tozeur
Centre Régional de Recherche en Agriculture Oasienne
Cerqueira, Frédérique
France, Montpellier
Institut Des Sciences de L’evolution de Montpellier
Drira, Noureddine
Tunisia, Sfax
Faculté Des Sciences de Sfax
Gharsallah, Néji
Tunisia, Sfax
Faculté Des Sciences de Sfax
Vallaeys, Tatiana
France, Sete
Marbec Marine Biodiversity, Exploitation and Conservation
Statistics
Citations: 21
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1631/jzus.B1200300
ISSN:
16731581
e-ISSN:
18621783
Research Areas
Genetics And Genomics