Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Correlation of in vitro platelet quality measurements with in vivo platelet viability in human subjects
Vox Sanguinis, Volume 90, No. 4, Year 2006
Notification
URL copied to clipboard!
Description
Background and Objectives: Changes in in vitro platelet quality parameters during platelet storage are associated with a decrease of in vivo platelet viability after platelet transfusion. Many attempts have been made to identify the most predictable in vitro parameters for in vivo performance. We used a riboflavin-based ultraviolet (UV) light treatment process designed to inactivate pathogens and white blood cell (WBC) contaminants in blood products as a model system in which to study the correlation of in vitro cell quality with in vivo viability. Materials and Methods: Platelet products (n = 18) were collected by a standard Trima apheresis procedure and treated with one of three dose levels of UV light (0, 7.2 or 12.4 J/ml) in the presence of 50 μm riboflavin. Lactate production, glucose consumption and P-selectin expression, pH, pCO2, pO2, hypotonic shock response and swirl were measured during 5 days of platelet storage post-UV/RB treatment. Aliquots of these products were radiolabelled on day 5 of storage and were subsequently used to determine platelet recovery and survival time in autologous subjects. Results: The responses of in vitro cell quality were observed to occur in a UV dose-dependent manner. Lactate production and pH were identified as the parameters most strongly correlated with platelet in vivo recovery, which ranged from 5 to 82%. The correlation coefficients (r) for lactate production and pH with in vivo recovery in human subjects were 0.9090 and 0.8831 with P-values of 0.007 and 0.031, respectively. Lactate production and pH were also found to be correlated with platelet survival time, with correlation coefficients of 0.8063 and 0.8384 (the P values were 0.01 and 0.001, respectively). Conclusions: Using conditions of riboflavin-based UV light treatment, lactate production and pH were identified as having the highest correlations with recovery and survival of radiolabelled platelets in healthy subjects. © 2006 Blackwell Publishing.
Authors & Co-Authors
Goodrich, Raymond P.
United States, Lakewood
Navigant Biotechnologies, Inc.
Li, Junzhi
United States, Lakewood
Navigant Biotechnologies, Inc.
Pieters, Henry
South Africa, Bloemfontein
University of the Free State, School of Medicine
Crookes, Robert L.
South Africa, Weltevreden Park
South African National Blood Service
Roodt, Jan P.
South Africa, Bloemfontein
University of the Free State, School of Medicine
Heyns, Anthon Du P.
South Africa, Weltevreden Park
South African National Blood Service
Statistics
Citations: 117
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1111/j.1423-0410.2006.00761.x
ISSN:
00429007