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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Effect of maternal preconceptional and pregnancy micronutrient interventions on children's DNA methylation: Findings from the EMPHASIS study
American Journal of Clinical Nutrition, Volume 112, No. 4, Year 2020
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Description
Background: Maternal nutrition in pregnancy has been linked to offspring health in early and later life, with changes to DNA methylation (DNAm) proposed as a mediating mechanism. Objective: We investigated intervention-associated DNAm changes in children whose mothers participated in 2 randomized controlled trials of micronutrient supplementation before and during pregnancy, as part of the EMPHASIS (Epigenetic Mechanisms linking Preconceptional nutrition and Health Assessed in India and sub-Saharan Africa) study (ISRCTN14266771). Design: We conducted epigenome-wide association studies with blood samples from Indian (n = 698) and Gambian (n = 293) children using the Illumina EPIC array and a targeted study of selected loci not on the array. The Indian micronutrient intervention was food based, whereas the Gambian intervention was a micronutrient tablet. Results: We identified 6 differentially methylated CpGs in Gambians [2.5-5.0% reduction in intervention group, all false discovery rate (FDR) <5%], the majority mapping to ESM1, which also represented a strong signal in regional analysis. One CpG passed FDR <5% in the Indian cohort, but overall effect sizes were small (<1%) and did not have the characteristics of a robust signature. We also found strong evidence for enrichment of metastable epialleles among subthreshold signals in the Gambian analysis. This supports the notion that multiple methylation loci are influenced by micronutrient supplementation in the early embryo. Conclusions: Maternal preconceptional and pregnancy micronutrient supplementation may alter DNAm in children measured at 7-9 y. Multiple factors, including differences between the nature of the intervention, participants, and settings, are likely to have contributed to the lack of replication in the Indian cohort. Potential links to phenotypic outcomes will be explored in the next stage of the EMPHASIS study. Copyright © The Author(s) on behalf of the American Society for Nutrition 2020.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC7528567/bin/nqaa193_supplemental_file.docx
Authors & Co-Authors
Saffari, Ayden
United Kingdom, London
Medical Research Council
Shrestha, Smeeta
India, Hyderabad
Centre for Cellular and Molecular Biology India
India, Bengaluru
Dayananda Sagar University
Issarapu, Prachand
India, Hyderabad
Centre for Cellular and Molecular Biology India
Sajjadi, Sara
India, Hyderabad
Centre for Cellular and Molecular Biology India
Betts, Modupeh
United Kingdom, London
London School of Hygiene & Tropical Medicine
Sahariah, Sirazul Ameen
India, Mumbai
Centre for the Study of Social Change
Tomar, Ashutosh Singh
India, Hyderabad
Centre for Cellular and Molecular Biology India
James, Philip T.
United Kingdom, London
Medical Research Council
Yadav, Dilip Kumar
India, Hyderabad
Centre for Cellular and Molecular Biology India
United States, Boston
Boston University
Kumaran, Kalyanaraman
United Kingdom, Southampton
University of Southampton
Prentice, Andrew M.
United Kingdom, London
Medical Research Council
United Kingdom, London
London School of Hygiene & Tropical Medicine
Lillycrop, Karen A.
United Kingdom, Southampton
University of Southampton
Fall, Caroline H.D.
United Kingdom, Southampton
University of Southampton
Ratan Chandak, Giriraj Ratan
India, Hyderabad
Centre for Cellular and Molecular Biology India
Silver, Matt J.
United Kingdom, London
Medical Research Council
Shrestha, Smeeta
Unknown Affiliation
James, Philip W.P.T.
Unknown Affiliation
Fall, Caroline H.D.
Unknown Affiliation
Cooper, Cyrus C.
Unknown Affiliation
Gravio, Chiara Di
Unknown Affiliation
Kehoe, Sarah Helen
Unknown Affiliation
Potdar, Ramesh Dattatray
Unknown Affiliation
Yajnik, Chittaranjan Sakerlal
Unknown Affiliation
Nongmaithem, Suraj Singh
Unknown Affiliation
Chopra, Harsha Vipin
Unknown Affiliation
Gandhi, Meera Jayant
Unknown Affiliation
Kaur, Lovejeet
Unknown Affiliation
Ngum, Mohammed
Unknown Affiliation
Darboe, Momodou K.
Unknown Affiliation
Goldberg, Gail R.
Unknown Affiliation
Acolatse, Lena
Unknown Affiliation
Owens, Stephen
Unknown Affiliation
Prentice, Ann M.
Unknown Affiliation
Ward, Kate Anna
Unknown Affiliation
Jarjou, Landing M.A.
Unknown Affiliation
Janha, Ramatoulie E.
Unknown Affiliation
Statistics
Citations: 14
Authors: 36
Affiliations: 7
Identifiers
Doi:
10.1093/ajcn/nqaa193
ISSN:
00029165
Research Areas
Food Security
Genetics And Genomics
Maternal And Child Health
Sexual And Reproductive Health
Study Design
Randomised Control Trial
Cohort Study