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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Aqueous extract of Nigella sativa seeds suppresses testicular steroidogenesis in mice leydig cells in vitro
Iranian Journal of Pharmacology and Therapeutics, Volume 12, No. 1, Year 2013
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Description
Nigella sativa (black seed) is an important medicinal herb with folkloric use in wide range of diseases. It is well studied for its biological activities. However, there is limited information regarding its effect on the male reproductive system. This study describes the effect of the aqueous extract of N. sativa (NSE) on testicular steroidogenesis from mice Leydig cells in vitro. Mice testicular cells were incubated in a media containing either no treatment or NSE or LH alone or combination of LH and NSE. Incubations were carried out for three hours in a shaking water bath at 34°C. Testosterone was measured by radioimmunoassay. At all doses, NSE significantly (p < 0.05) inhibited both basal and LH-stimulated in vitro testosterone secretion. At a dose of l000 μg, NSE inhibited 52% of basal testosterone and 97% of LH-stimulated testosterone, compared to control (0.32 ± 0.008 ng/ml) and LH alone (0.33 ± 0.01 ng/ml) respectively. Thus, it is concluded that that both the basal and the LH-stimulated secretion of testosterone from Leydig cells are suppressed significantly in the presence of different doses of NSE in vitro. However, further studies are needed to explore the effect of chronic treatment with NSE in male and its potential to be used as a contraceptive in male. © 2013 by Tehran University of Medical Sciences (TUMS).
Authors & Co-Authors
Saeed, Sheikh Abdul
Saudi Arabia, Riyadh
King Saud Bin Abdulaziz University for Health Sciences
Anwar, Naheed
Pakistan, Karachi
The Aga Khan University
Jabeen, Qaiser
Pakistan, Karachi
The Aga Khan University
Gilani, Anwar ul Hassan
Pakistan, Karachi
The Aga Khan University
Statistics
Citations: 4
Authors: 4
Affiliations: 2
Identifiers
ISSN:
17352657
Research Areas
Environmental
Sexual And Reproductive Health
Participants Gender
Male