Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Analysis of the diversity of the HIV-1 pol gene and drug resistance associated changes among drug-naïve patients in Burkina Faso
Journal of Medical Virology, Volume 81, No. 10, Year 2009
Notification
URL copied to clipboard!
Description
A cross-sectional study was undertaken among drug-naïve HIV patients at the University Hospital in Ouagadougou shortly before and after the introduction of large-scale antiretroviral therapy (ART) in Burkina Faso. Baseline clinical and virological data as well as protease (PR) and 5′ reverse transcriptase (RT) sequences from 104 HIV infected patients were analyzed. Genotypic classification revealed the following subtypes and recombinant forms: CRF06-cpx, n = 46 (44.2%); CRF02-AG, n = 39 (37.5%); subtype A, n = 4 (3.8%); CRF09-cpx, n = 2 (1.9%); and unclassified, n = 13 (12.5%). Bootstrap analysis of CRF02-AG and CRF06-cpx viruses showed that >80% had a similar structure to their respective prototypes. The prevalence of primary drug resistance mutations was 12.5%, all mutations arising in the RT sequences in accordance with the dominance of this drug class in Burkina Faso. The mutations were distributed as follows: NRTI (10.6%): M41L (n = 2), D67N (n = 2), K70K/E (n = 2), L210W (n = 1), T215S/Y (n = 2), and K219K/Q (n = 2); NNRTI (6.1%): K103K/N (n = 2), Y181C (n = 2), G190G/A (n = 1), and P236P/L (n = 1). Subtype specific secondary polymorphisms such as K20I and M36I in the PR were observed in almost all patients. Drug resistance mutations occurred at similar frequencies (12.8% and 10.8%, respectively) among patients infected with CRF02-AG and CRF06-cpx. Some subtype specific polymorphisms were observed within important HLA epitopes, including B35, B7, and A2 in the RT, and A*6802 in the PR sequences. The observed resistance mutations are most likely to have been transmitted based on the timing of the study but prior undocumented use of ART cannot be excluded. © 2009 Wiley-Liss, Inc.
Authors & Co-Authors
Tebit, Denis Manga
Germany, Heidelberg
Universitätsklinikum Heidelberg
United States, Cleveland
Case Western Reserve University
Sangaré, Lassana
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Tiba, Fabrice
Germany, Heidelberg
Universitätsklinikum Heidelberg
Saydou, Yameogo
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Makamtse, Aline
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Somlaré, Hermann
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Bado, Guillaume
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Kouldiaty, Boris G.
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Zabsonre, Inoussa
Burkina Faso, Ouagadougou
Universitaire Yalgado-ouédraogo
Yaméogo, S. L.C.
Burkina Faso, Ouagadougou
Universitaire Yalgado-ouédraogo
Sathiandee, Kanokporn
Germany, Heidelberg
Universitätsklinikum Heidelberg
Drabo, Joseph Youssouf
Burkina Faso, Ouagadougou
Universitaire Yalgado-ouédraogo
Kraüsslich, Hans Georg
Germany, Heidelberg
Universitätsklinikum Heidelberg
Statistics
Citations: 37
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.1002/jmv.21600
ISSN:
01466615
e-ISSN:
10969071
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Burkina Faso