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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Molecular identification of Knops blood group polymorphisms found in long homologous region D of complement receptor 1
Blood, Volume 97, No. 9, Year 2001
Notification
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Description
Complement receptor 1 (CR1) has been implicated in rosetting of uninfected red blood cells to Plasmodium falciparuminfected cells, and rosette formation is associated with severe malaria. The Knops blood group (KN) is located on CR1 and some of these antigens, ie, McCoy (McC) and Swain-Langley (Sla), show marked frequency differences between Caucasians and Africans. Thus, defining the molecular basis of these antigens may provide new insight into the mechanisms of P falciparum malaria. Monoclonal antibody epitope mapping and serologic inhibition studies using CR1 deletion constructs localized McC and Sla to long homologous repeat D of CR1. Direct DNA sequencing of selected donors identified several single nucleotide polymorphisms in exon 29 coding for complement control protein modules 24 and 25. Two of these appeared to be blood group specific: McC associated with K1590E and Sla with R1601G. These associations were confirmed by inhibition studies using allelespecific mutants. A sequence-specific oligonucleotide probe hybridization assay was developed to genotype several African populations and perform family inheritance studies. Concordance between the 1590 mutation and McC was 94%; that between Sla and 1601 was 88%. All but 2 samples exhibiting discrepancies between the genotype and phenotype were found to be due to low red cell CR1 copy numbers, low or absent expression of some alleles, or heterozygosity combined with low normal levels of CR1. These data further explain the variability observed in previous serologic studies of CR1 and show that DNA and proteinbased genetic studies will be needed to clarify the role of the KN antigens in malaria. © 2001 by The American Society of Hematology.
Authors & Co-Authors
Moulds, Joann M.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
United States, Philadelphia
Drexel University College of Medicine
Zimmerman, Peter A.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Doumbo, Ogobara K.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Kassambara, L.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Sagara, Issaka
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Diallo, Dapa Aly
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Atkinson, John P.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Krych-Goldberg, Malgorzata
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Hauhart, Richard
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Hourcade, Dennis E.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
McNamara, David T.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Birmingham, Daniel Joseph
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Rowe, Jane Alexandra
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Moulds, John J.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Miller, Louis H.
United States, Houston
University of Texas Medical School at Houston
United States, Cleveland
Case Western Reserve University
Mali, Bamako
University of Bamako
United States, St. Louis
Washington University School of Medicine in St. Louis
United States, Columbus
The Ohio State University
United Kingdom, Edinburgh
The University of Edinburgh
United States, Raritan
Ortho-mcneil Pharmaceutical Incorporated
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 82
Authors: 15
Affiliations: 9
Identifiers
Doi:
10.1182/blood.V97.9.2879
ISSN:
00064971
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases