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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Effects of peroxynitrite derived from nitric oxide on cultured bovine ocular explants
Journal Francais d'Ophtalmologie, Volume 36, No. 1, Year 2013
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Description
Introduction: Several studies have reported a significant production of nitric oxide (NO) with peroxynitrite formation in the setting of intraocular inflammation. In a previous study, we showed the cytotoxic effect of nitrites and nitrates, stable metabolites of NO, on the various tissues forming the layers of the eye, with variable degrees of tissue sensitivity. This study aims to investigate the effect of peroxynitrite on whole ocular bovine explants in culture. Methods: Healthy ocular bovine eyes, obtained immediately upon enucleation, were dissected and samples were taken from the anterior and posterior segments, and then cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine and antibiotics. Cultures were treated with 3-morpholino-sydonimin N-ethyl-carbamide (SIN-1) (molecule which produces NO and superoxide anion O2.-) at varying concentrations (100 to 500 μM) over 24 hours. After incubation, the explants were fixed in 10% buffered formalin, and histological study was performed. Results: Most of the structures showed changes on tissue and cellular levels after incubation with the peroxynitrite donor and various responses depending on the concentration used. These observations reflect variable concentration-dependent tissue sensitivity. The epithelia (cornea, iris and ciliary process) showed high sensitivity in comparison with sclera, which developed greater resistance. Conclusion: In all, our results indicate a deleterious effect of peroxynitrite on bovine ocular structures in vitro. This effect is proportional to the concentration used. These results corroborate those reported by other teams and suggest the role of peroxynitrite derived from NO in the ocular lesions observed in the setting of uveitis. © 2012 Publié par Elsevier Masson SAS.
Authors & Co-Authors
Lahmar-Belguendouz, Karima
Algeria, Algiers
Université Des Sciences et de la Technologie Houari Boumediene
Belguendouz, Houda
Algeria, Algiers
Université Des Sciences et de la Technologie Houari Boumediene
Hartani, Dahbia
Algeria, Algiers
Centre Hospitalo-universitaire de Mustapha Bacha
Lahlou-Boukoffa, Ouidad S.
Algeria, Annaba
Centre Hospitalier Universitaire de Annaba
Bédiar-Boulaneb, F.
Algeria, Annaba
Centre Hospitalier Universitaire de Annaba
Touil-Boukoffa, Chafia
Algeria, Algiers
Université Des Sciences et de la Technologie Houari Boumediene
Statistics
Citations: 6
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.jfo.2012.04.009
ISSN:
01815512
Research Areas
Health System And Policy