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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A standardised protocol for the evaluation of small extracellular vesicles in plasma by imaging flow cytometry
Journal of Immunological Methods, Volume 468, Year 2019
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Description
Flow cytometry provides robust, multi-parametric and quantitative information on single cells which also exhibits enormous potential as a tool for small particle characterisation. Small extracellular vesicle (sEV) detection by flow cytometry remains compromised due to the high prevalence of swarm detection, which is defined by the simultaneous illumination of more than one sEV, recorded as a single event. Detection of sEVs by imaging flow cytometry presents a major advantage by having the ability to resolve single particles from swarm detection based on the image features recorded for each event. In this study, we provide a simplified protocol that facilitates the removal of both swarm events and aggregated particles to improve the accuracy of sEV analysis. Our results indicate that imaging flow cytometry should be at the forefront as a robust and sensitive technique for sEV characterisation. © 2019 Elsevier B.V.
Authors & Co-Authors
Gray, Elin Solomonovna
Australia, Perth
Edith Cowan University
Moodley, Yuben P.
Australia, Perth
The University of Western Australia
Australia, Perth
Institute for Respiratory Health
Australia, Perth
Fiona Stanley Hospital
Statistics
Citations: 6
Authors: 2
Affiliations: 5
Identifiers
Doi:
10.1016/j.jim.2019.03.006
ISSN:
00221759
Study Design
Cross Sectional Study
Study Approach
Quantitative