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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A field-adapted HPLC method for determination of amodiaquine and its metabolite in whole blood dried on filter paper
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Volume 859, No. 1, Year 2007
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Description
A reversed-phase high performance liquid chromatographic method was developed and validated for the quantitative determination of amodiaquine (AQ) and its metabolite desethylamodiaquine (DAQ) in whole blood collected on filter paper. The structure analogue 4-(4-dimethylamino-1-methylbutylamino)-7-chloroquinoline was used as internal standard. Upon collection, blood was added to 10% phosphoric acid in a 1:1 ratio and then spotted onto filter paper. The samples were alkalinized (pH ≈ 9.2) with potassium hydroxide at the time of assay and the compounds were extracted together with internal standard into di-isopropyl ether and then re-extracted into an aqueous phase with 0.1 M phosphate buffer at pH 4. The chromatographic analysis was performed using an Agilent Technologies ChemStation LC System. The absorbance of the compounds was monitored at 333 nm. Mean extraction recoveries of AQ and DAQ were 49 and 48%, respectively. Intra-day and inter-day coefficients of variation were <10.5%. The limit of quantification was 50 nM for both compounds (sample size 100 μl). Both AQ and DAQ that were previously reported to be unstable have been stored on filter paper for at least 19 weeks. The method was applied on samples from healthy volunteers. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Ntale, Muhammad
Uganda, Kampala
Makerere University
Mahindi, Margarita
Unknown Affiliation
Ogwal-Okeng, Jasper Watson
Uganda, Kampala
Makerere University
Gustafsson, Lars L.
Unknown Affiliation
Beck, Olof M.L.
Unknown Affiliation
Statistics
Citations: 35
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1016/j.jchromb.2007.09.012
ISSN:
15700232
Study Approach
Quantitative