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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Methods for derivation and detection of anti-parasite monoclonal antibodies
Journal of Immunological Methods, Volume 34, No. 2, Year 1980
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Description
We describe detailed methods for derivation and cloning of myeloma hybrids which secrete antibodies specific for antigens of protozoan parasites. The methods were designed to enable the derivation of large numbers of specific monoclonal antibodies and to give high cloning efficiencies of desired hybrids. Although special attention is paid to derivation and detection of anti-parasite antibodies, the methods can be applied to many different antibody-antigen systems. Using the described methods we have isolated more than 90 myeloma hybrids which secrete antibodies specific for antigens of African trypanosomes and Theileria parasites, thus illustrating their effectiveness. © 1980.
Authors & Co-Authors
Pearson, Terry W.
Unknown Affiliation
Pinder, Margaret
Unknown Affiliation
Roelants, Georges E.
Unknown Affiliation
Kar, Santosh Kumar
Unknown Affiliation
Lundin, Lena B.
Unknown Affiliation
Mayor-Withey, Kathleen S.
Unknown Affiliation
Hewett, Rosemary S.
Unknown Affiliation
Statistics
Citations: 79
Authors: 7
Identifiers
Doi:
10.1016/0022-1759(80)90168-4
ISSN:
00221759