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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Long-term storage at tropical temperature of dried-blood filter papers for detection and genotyping of RNA and DNA viruses by direct PCR
Journal of Virological Methods, Volume 146, No. 1-2, Year 2007
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Description
In tropical countries the diagnosis of viral infections of humans or animals is often hampered by the lack of suitable clinical material and the necessity to maintain a cold chain for sample preservation up to the laboratory. This study describes the use of filter papers for rapid sample collection, and the molecular detection and genotyping of viruses when stored over long periods at elevated temperatures. Infected blood was collected on filter papers, dried and stored at different temperatures (22, 32 and 37 °C) for various periods (up to 9 months). Two animal viruses, African swine fever, a large double-stranded DNA virus and Peste des Petits Ruminants, a negative single-stranded RNA virus, were used to validate the method. Filter papers with dried blood containing virus or control plasmid DNA were cut in small 5 mm2 pieces and added directly to the PCR tube for conventional PCR. Nucleic acid from both viruses could still be detected after 3 months at 32 °C. Moreover, the DNA virus could be detected at least 9 months after conservation at 37 °C. PCR products obtained from the filter papers were sequenced and phylogenetic analysis carried out. The results were consistent with published sequences, demonstrating that this method can be used for virus genotyping. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Michaud, Vincent
France, Paris
Cirad
Gil, Patricia
France, Paris
Cirad
Kwiatek, Olivier
France, Paris
Cirad
Prome, S.
France, Paris
Cirad
Dixon, Linda K.
United Kingdom, Surrey
The Pirbright Institute
Romero, Luis J.
Spain, Valdeolmos
Csic-inia-cisa - Centro de Investigación en Sanidad Animal
Le Potier, Marie Frédérique L.E.
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Arias, M.
Spain, Valdeolmos
Csic-inia-cisa - Centro de Investigación en Sanidad Animal
Couacy-Hymann, Emmanuel
Cote D'ivoire
Laboratoire Central de Pathologie Animale
Roger, François Louis
France, Paris
Cirad
Libeau, Geneviève
France, Paris
Cirad
Albina, Emmanuel
France, Paris
Cirad
Statistics
Citations: 66
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1016/j.jviromet.2007.07.006
ISSN:
01660934
Research Areas
Genetics And Genomics