Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Variability in the precore and core promoter regions of HBV strains in morocco: Characterization and impact on liver disease progression
PLoS ONE, Volume 7, No. 8, Article e42891, Year 2012
Notification
URL copied to clipboard!
Description
Background: Hepatitis B virus (HBV) is one of the most common human pathogens that cause aggressive hepatitis and advanced liver disease (AdLD), including liver cirrhosis and Hepatocellular Carcinoma. The persistence of active HBV replication and liver damage after the loss of hepatitis B e antigen (HBeAg) has been frequently associated with mutations in the pre-core (pre-C) and core promoter (CP) regions of HBV genome that abolish or reduce HBeAg expression. The purpose of this study was to assess the prevalence of pre-C and CP mutations and their impact on the subsequent course of liver disease in Morocco. Methods/Principal Findings: A cohort of 186 patients with HBeAg-negative chronic HBV infection was studied (81 inactive carriers, 69 with active chronic hepatitis, 36 with AdLD). Pre-C and CP mutations were analyzed by PCR-direct sequencing method. The pre-C stop codon G1896A mutation was the most frequent (83.9%) and was associated with a lower risk of AdLD development (OR, 0.4; 95% CI, 0.15-1.04; p = 0.04). HBV-DNA levels in patients with G1896A were not significantly different from the other patients carrying wild-type strains (p = 0.84). CP mutations C1653T, T1753V, A1762T/G1764A, and C1766T/T1768A were associated with higher HBV-DNA level and increased liver disease severity. Multiple logistic regression analysis showed that older age (≥40 years), male sex, high viral load (>4.3 log10 IU/mL) and CP mutations C1653T, T1753V, A1762T/G1764A, and C1766T/T1768A were independent risk factors for AdLD development. Combination of these mutations was significantly associated with AdLD (OR, 7.52; 95% CI, 4.8-8; p<0.0001). Conclusions: This study shows for the first time the association of HBV viral load and CP mutations with the severity of liver disease in Moroccan HBV chronic carriers. The examination of CP mutations alone or in combination could be helpful for prediction of the clinical outcome. © 2012 Kitab et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3419231/bin/pone.0042891.s001.doc
Authors & Co-Authors
Kitab, Bouchra
Morocco, Casablanca
Institut Pasteur du Maroc
Morocco, Casablanca
Faculté Des Sciences Ben M’sick
Essaid El Feydi, Abdellah
Morocco, Agdal Rabat
Ibn Sina Hospital, Agdal Rabat
Afifi, Rajae
Morocco, Agdal Rabat
Ibn Sina Hospital, Agdal Rabat
Trépo, Christian R.
France, Lyon
Centre de Recherche en Cancérologie de Lyon
Benazzouz, Moustapha
Morocco, Agdal Rabat
Ibn Sina Hospital, Agdal Rabat
Essamri, Wafaa
Morocco, Agdal Rabat
Ibn Sina Hospital, Agdal Rabat
Zoulim, Fabien
France, Lyon
Centre de Recherche en Cancérologie de Lyon
Chemin, Isabelle A.
France, Lyon
Centre de Recherche en Cancérologie de Lyon
Alj, Hanane Salih
Morocco, Casablanca
Faculté Des Sciences Ben M’sick
Ezzikouri, Sayeh
Morocco, Casablanca
Institut Pasteur du Maroc
Benjelloun, Soumaya
Morocco, Casablanca
Institut Pasteur du Maroc
Statistics
Citations: 40
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pone.0042891
e-ISSN:
19326203
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Cohort Study
Study Approach
Quantitative
Study Locations
Morocco
Participants Gender
Male