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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies
Journal of Clinical Microbiology, Volume 51, No. 2, Year 2013
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Description
Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spotted on protein microarrays and screened using different lukF-PV preparations and detection antibodies. This led to the identification of the optimal antibody combination that was then used to establish a lateral flow assay. This test was used to detect PVL in S. aureus cultures. The detection limit of the assay with purified native and recombinant antigens was determined to be around 1 ng/ml. Overnight cultures from various solid and liquid media proved suitable for PVL detection. Six hundred strains and clinical isolates from patients from America, Europe, Australia, Africa, and the Middle East were tested. Isolates were genotyped in parallel by DNA microarray hybridization for confirmation of PVL status and assignment to clonal complexes. The sensitivity, specificity, and positive and negative predictive values of the assay in this trial were 99.7, 98.3, 98.4, and 99.7%, respectively. A total of 302 clinical isolates and reference strains were PVL positive and were assigned to 21 different clonal complexes. In summary, the lateral flow test allows rapid and economical detection of PVL in a routine bacteriology laboratory. As the test utilizes cultures from standard media and does not require sophisticated equipment, it can be easily integrated into a laboratory's workflow and might contribute to timely therapy of PVLassociated infections. Copyright © 2013, American Society for Microbiology.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3553917/bin/supp_51_2_487__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3553917/bin/JCM.02285-12_zjm999092258so1.pdf
Authors & Co-Authors
Monecke, Stefan
Germany, Jena
Alere Technologies Gmbh
Germany, Dresden
Technische Universität Dresden
Müller, Elke
Germany, Jena
Alere Technologies Gmbh
Buechler, Joseph
United States, San Diego
Alere San Diego, Inc.
Rejman, John
United States, Scarborough
Alere Scarborough Inc.
Stieber, Bettina
Germany, Jena
Alere Technologies Gmbh
Germany, Dresden
Technische Universität Dresden
Akpaka, Patrick Eberechi
Trinidad and Tobago, St Augustine
The University of the West Indies, St. Augustine Campus
Bandt, Dirk
Germany, Frankfurt am Main
Institute for Medical Diagnostics Oderland
Burris, Rob
Uganda
Alere Technologies Gmbh
Coombs, Geoffrey W.
Australia, Perth
Curtin University
Aida Hidalgo-Arroyo, G.
United Kingdom, Bristol
Southmead Hospital Bristol
Hughes, Peter
Uganda, Kampala
Medical Research Council
Kearns, Angela Marie
United Kingdom, London
Public Health England
Abós, Sonia Molinos
Spain, Badalona
Hospital Universitari Germans Trias I Pujol
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Pichon, Bruno
United Kingdom, London
Public Health England
Skakni, Leila
Saudi Arabia, Riyadh
King Fahad Medical City
Söderquist, Bo
Sweden, Orebro
Universitetssjukhuset Örebro
Ehricht, Ralf
Germany, Jena
Alere Technologies Gmbh
Statistics
Citations: 38
Authors: 17
Affiliations: 15
Identifiers
Doi:
10.1128/JCM.02285-12
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics