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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Immuno-chromatographic wicking assay for the rapid detection of dengue viral antigens in mosquitoes (Diptera: Culicidae)
Journal of Medical Entomology, Volume 51, No. 1, Year 2014
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Description
There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) and Aedes albopictus (Skuse), are readily available. However, there are currently no accurate and reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. To detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 30 min. It was highly specific and did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas. © 2014 Entomological Society of America.
Authors & Co-Authors
Wanja, Elizabeth W.
Kenya
Us Army Medical Research Unit
United States, Silver Spring
Walter Reed Army Institute of Research
Parker, Zahra F.
United States, Philadelphia
University of Pennsylvania Perelman School of Medicine
Odusami, Oluwakemi
United States, Silver Spring
Walter Reed Army Institute of Research
United States, Baltimore
University of Maryland School of Law
Rowland, Tobin
United States, Silver Spring
Walter Reed Army Institute of Research
Davé, Kirti
United States, Thousand Oaks
Vector Test Systems, Inc
Davé, Sonia
United States, Thousand Oaks
Vector Test Systems, Inc
Turell, Michael J.
United States, Fort Detrick
U.s. Army Medical Research Institute of Infectious Diseases
Statistics
Citations: 7
Authors: 7
Affiliations: 6
Identifiers
Doi:
10.1603/ME12261
ISSN:
00222585
Research Areas
Infectious Diseases
Participants Gender
Female