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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Phenotypic conversion of cultured mouse embryo cells by aza pyrimidine nucleosides
Developmental Biology, Volume 66, No. 1, Year 1978
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Description
Cells of the C3H 10T 1 2CL8 line, which are nonmyoblastic in nature, form functional myotubes when treated with low concentrations of 5-azacytidine. Further characterization of the myotubes revealed that they arise from the fusion of mononucleated precursors and not as a result of endoreplication. They accumulate histochemically detectable myosin ATPase activity as well as acetylcholine receptors capable of binding radioactively labeled α-bungarotoxin. The deoxy analog, 5-aza-2′-deoxycytidine, induced myogenic conversion at one-tenth of the maximally effective concentration of 5-azacytidine. The ability of both analogs to induce myotube formation and to cause cytotoxicity was strongly influenced by cotreatment with certain pyrimidine nucleosides. These effects were consistent with a requirement for metabolism of both aza compounds to phosphorylated derivatives and with a mechanism of action based on their incorporation into DNA. Concentrations of the analogs causing myogenic conversion did not substantially alter rates of DNA, RNA, or protein synthesis as measured by precursor incorporation into intact cells. The induction of myotubes by 5-azacytidine in cells synchronized by two different methods required that treatment with the analog was carried out at a critical phase early in S phase. Thus the mechanism of drug action appears to be linked to specific DNA synthesis. © 1978.
Authors & Co-Authors
Constantinides, Philip G.
South Africa, Stellenbosch
Stellenbosch University
Taylor, S. M.
South Africa, Stellenbosch
Stellenbosch University
Jones, Peter A.
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 138
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/0012-1606(78)90273-7
ISSN:
00121606
Research Areas
Genetics And Genomics