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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance
Microbiology (United Kingdom), Volume 158, No. 9, Year 2012
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Description
Burkholderia cenocepacia is a member of the Burkholderia cepacia complex (Bcc), a group of Gram-negative opportunistic pathogens that cause severe lung infections in patients with cystic fibrosis and display extreme intrinsic resistance to antibiotics, including antimicrobial peptides. B. cenocepacia BCAL2157 encodes a protein homologous to SuhB, an inositol-1-monophosphatase from Escherichia coli, which was suggested to participate in post-transcriptional control of gene expression. In this work we show that a deletion of the suhB-like gene in B. cenocepacia (ΔsuhBBc) was associated with pleiotropic phenotypes. The ΔsuhBBc mutant had a growth defect manifested by an almost twofold increase in the generation time relative to the parental strain. The mutant also had a general defect in protein secretion, motility and biofilm formation. Further analysis of the type II and type VI secretion systems (T2SS and T6SS) activities revealed that these secretion systems were inactive in the ΔsuhBBc mutant. In addition, the mutant exhibited increased susceptibility to polymyxin B but not to aminoglycosides such as gentamicin and kanamycin. Together, our results demonstrate that suhBBc deletion compromises general protein secretion, including the activity of the T2SS and the T6SS, and affects polymyxin B resistance, motility and biofilm formation. The pleiotropic effects observed upon suhBBc deletion demonstrate that suhBBc plays a critical role in the physiology of B. cenocepacia. © 2012 SGM.
Authors & Co-Authors
Rosales-Reyes, Roberto
Canada, London
Western University
Mexico, Mexico
Universidad Nacional Autónoma de México, Facultad de Medicina
Saldías, María Soledad
Canada, London
Western University
Aubert, Daniel F.
Canada, London
Western University
El-Halfawy, Omar M.
Canada, London
Western University
Egypt, Alexandria
Faculty of Pharmacy
Valvano, Miguel Ángel
Canada, London
Western University
Statistics
Citations: 21
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1099/mic.0.060988-0
ISSN:
13500872
Research Areas
Genetics And Genomics