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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Ser/Thr/Tyr phosphoproteome characterization of Acinetobacter baumannii: Comparison between a reference strain and a highly invasive multidrug-resistant clinical isolate
Journal of Proteomics, Volume 102, Year 2014
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Description
In the current study, the Ser/Thr/Tyr phosphoproteomes of two Acinetobacter baumannii strains, reference (ATCC17978) and highly invasive multidrug-resistant clinical isolate (Abh12O-A2) were analyzed using SCX and TiO2 chromatography followed by high resolution mass spectrometry. We detected a total of 201 unique phosphorylation sites (p-sites), and, after manual validation of peptide spectra, 91 high-confidence phosphorylation events (p-events) could be localized to a specific amino acid residue. The percentage distribution of Ser/Thr/Tyr phosphorylation was 68.9% on serine, 24.1% on threonine and 5.2% on tyrosine in ATCC17978, and 70.8% on serine, 25.2% on threonine and 3.8% on tyrosine in AbH12O-A2. Across all identified p-sites, 11 were identified in ATCC17978 only, while 43 were identified in Abh12O-A2 only, and 37 overlapped between the two strains. Here for the first time we describe the phosphoproteome of A. baumanii, and significance of selected phosphorylation sites is discussed in the context of stress/starvation, pathogenicity and drug resistance. Biological significance: It is now well established that protein phosphorylation on Ser/Thr/Tyr residues is an important post-translational modification in bacteria. Herein we employed SCX and TiO2 chromatographic phosphopeptide enrichment combined with LTQ-Orbitrap mass spectrometric analyses to characterize and establish a qualitative comparison between the Ser/Thr/Tyr phosphoproteomes of two Acinetobacter baumannii strains: a reference strain and a highly invasive multidrug-resistant clinical isolate. We highlight the identification of phosphoproteins with a role in pathogenicity and those involved in drug resistance. © 2014 Elsevier B.V.
Authors & Co-Authors
Soares, Nelson C.
Germany, Tubingen
Eberhard Karls Universität Tübingen
Spain, Corunna
Complejo Hospitalario Universitario Juan Canalejo
South Africa, Cape Town
Faculty of Health Sciences
Spät, Philipp
Germany, Tubingen
Eberhard Karls Universität Tübingen
Méndez, José Antonio
Spain, Corunna
Complejo Hospitalario Universitario Juan Canalejo
Nakedi, Kehilwe C.
South Africa, Cape Town
Faculty of Health Sciences
Aranda, Jesús
Spain, Corunna
Complejo Hospitalario Universitario Juan Canalejo
Bou, Germán
Spain, Corunna
Complejo Hospitalario Universitario Juan Canalejo
Statistics
Citations: 41
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.jprot.2014.03.009
ISSN:
18743919
e-ISSN:
18767737
Study Approach
Qualitative