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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Expedient liquid chromatographic method with fluorescence detection for montelukast sodium in micro-samples of plasma
Journal of Chromatography B: Biomedical Sciences and Applications, Volume 754, No. 2, Year 2001
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Description
This study describes an expedient assay for the analysis of the asthma medication, montelukast sodium (Singulair, MK-0476), in human plasma samples. After a simple extraction of the plasma, the drug and internal standard, quinine bisulfate, were measured by HPLC. The chromatographic system consisted of a single pump, a refrigerated autosampler, a C8 4-μm particle size radial compression cartridge at 40°C and a fluorescence detector with the excitation and emission wavelengths set at 350 and 400 nm, respectively. The mobile phase which was delivered at 1.0 ml/min, was prepared by adding 200 ml of 0.025 M sodium acetate, pH adjusted to 4.0 with acetic acid, to 800 ml of acetonitrile, with 50 μl triethylamine. With a run time of only 10 min per sample, this assay had an overall recovery of >97% with a detection limit of 1 ng/ml. The inter- and intra-run relative standard deviations at 0.05, 0.2 and 1.0 μg/ml were all <9.2%, while the analytical recovery at the same concentrations were within 7.7% of the amount added. © 2001 Elsevier Science B.V.
Authors & Co-Authors
Al-Rawithi, Sameer
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Al-Gazlan, Sulaiman S.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
al-Ahmadi, Wijdan
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Alshowaier, Ibrahim A.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Yusuf, Ahmed
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Raines, Dale A.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Statistics
Citations: 52
Authors: 6
Affiliations: 1
Identifiers
Doi:
10.1016/S0378-4347(01)00025-1
ISSN:
13872273
Research Areas
Environmental