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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Restoration of mycobacterial antigen-induced proliferation and interferon-γ responses in peripheral blood mononuclear cells of tuberculosis patients upon effective chemotherapy
FEMS Immunology and Medical Microbiology, Volume 38, No. 3, Year 2003
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Description
Peripheral blood mononuclear cells (PBMC) were obtained from culture-proven tuberculosis (TB) patients before and after 2 and 6 months of chemotherapy with a multi-drug regimen. PBMC were tested for cellular responses in antigen-induced proliferation and interferon-γ (IFN-γ) assays in response to complex mycobacterial antigens (whole cell Mycobacterium bovis BCG and M. tuberculosis, cell walls and short-term culture filtrate [ST-CF] of M. tuberculosis), fractionated ST-CF antigens (fractions F1-F10) and ESAT-6. The responses in TB patients before anti-TB treatment were low (median stimulation index (SI)=1-7, median delta IFN-γ=0-12 U ml-1, and percent responders=13-67%) to all the antigenic preparations. Following the administration of anti-TB chemotherapy for 2 months, there were significant (P<0.05) improvements in the cellular responses (median SI=9-76, median delta IFN-γ=3-70 U ml-1, and percent responders=33-100%) to most of the antigenic preparations tested. However, concanavalin A-induced proliferation responses of PBMC from the same patients before and after 2 months of chemotherapy were high and comparable (median SI=101 and 114, respectively, P>0.05, 100% responders). A further increase in IFN-γ responses (median delta IFN-γ=14-250 U ml-1 and percent responders=43-100%) to mycobacterial antigens was observed in patients receiving chemotherapy for 6 months. Among the ST-CF fractions, F1 and F2 containing low molecular mass proteins resulted in the highest responses, whereas ESAT-6 showed responses comparable to these fractions only in a minority of the patients. HLA-DR typing of these patients showed heterogeneity in the expression of molecules encoded by HLA-DRB genes. These results show that effective chemotherapy restores cellular responses of TB patients to a large number of M. tuberculosis antigens, which could be useful in monitoring the efficacy of anti-TB treatment. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Authors & Co-Authors
Al-Attiyah, Raja'a J.
Kuwait, Kuwait City
Kuwait University
Mustafa, Abu Salim
Kuwait, Kuwait City
Kuwait University
Abal, Adnan T.
Kuwait, Kuwait City
Kuwait University
Madi, Nada Mohammed
Kuwait, Kuwait City
Kuwait University
Andersen, Peter Lawætz
Denmark, Copenhagen
Statens Serum Institut
Statistics
Citations: 80
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/S0928-8244(03)00166-4
ISSN:
09288244
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases