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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Suppressor mutagenesis identifies a velvet complex remediator of Aspergillus nidulans secondary metabolism
Eukaryotic Cell, Volume 9, No. 12, Year 2010
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Description
Fungal secondary metabolites (SM) are bioactive compounds that are important in fungal ecology and, moreover, both harmful and useful in human endeavors (e.g., as toxins and pharmaceuticals). Recently a nuclear heterocomplex termed the Velvet complex, characterized in the model ascomycete Aspergillus nidulans, was found to be critical for SM production. Deletion of two members of the Velvet complex, laeA and veA, results in near loss of SM and defective sexual spore production in A. nidulans and other species. Using a multicopy-suppressor genetics approach, we have isolated an Aspergillus nidulans gene named rsmA (remediation of secondary metabolism) based upon its ability to remediate secondary metabolism in ΔlaeA and ΔveA backgrounds. Overexpression of rsmA (OE::rsmA) restores production of sterigmatocystin (ST) (a carcinogenic SM) via transcriptional activation of ST biosynthetic genes. However, defects in sexual reproduction in either ΔlaeA or ΔveA strains cannot be overcome by OE::rsmA. An intact Velvet complex coupled with an OE::rsmA allele increases SM many fold over the wild-type level, but loss of rsmA does not decrease SM. RsmA encodes a putative bZIP basic leucine zipper-type transcription factor. © 2010, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3008278/bin/supp_9_12_1816__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3008278/bin/supp_9_12_1816__Tables_S1_and_S2.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC3008278/bin/supp_9_12_1816__Rev_Supp.ppt
Authors & Co-Authors
Shaaban, M. I.
United States, Madison
University of Wisconsin-madison
Egypt, Mansoura
Mansoura University
Bok, Jin Woo
United States, Madison
University of Wisconsin-madison
Lauer, Carrie
United States, Madison
University of Wisconsin-madison
Keller, Nancy P.
United States, Madison
University of Wisconsin-madison
Statistics
Citations: 83
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1128/EC.00189-10
ISSN:
15359778
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health