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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
IL-2 promoter-driven lacZ expression as a monitoring tool for IL-2 expression in primary T cells of transgenic mice
International Immunology, Volume 6, No. 2, Year 1994
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Description
A transgenic mouse system has been established to follow the pattern of IL-2 expression at the level of single T cells. This was achieved by introducing a human IL-2 promoter-driven reporter gene (Escherfchla coll lacZ) into the germllne of mice and monitoring Its product, β-galactosldase (β-gal), by FACS analysis. Ex vivo experiments confirmed that the regulated expression of the transgene Is comparable with that of the endogenous IL-2 gene. Transgene expression is induclble by mltogens, restricted to T cells, and diminished by Immunosuppressive agents, such as cyclosporln A, at concentrations known to suppress IL-2 transcription. Depending on the mltogens used, 30-50% of peripheral T cells produced IL-2 with an asynchronous induction pattern, as measured by transgenic β-gal activity. Both helper (CD4+CD8-) and cytotoxlc T cells (CD4-CD8+) respond with comparable heterogeneous expression levels but they show different frequencies of β-gal production. Transgenic β-gal-produclng T cells were detectable as early as 2 h after mitogen stimulation. These cells represent a transitional IL-2 secreting, IL-2 receptor α-chaJn negative T cell population, which occurs in the aftocrine process of T cell activation. Administration of staphytococcal enterotoxln A (SEA), a bacterial superantlgen, resulted in a T cell specific (Thy-1.2) increase (2.5-fold) of reporter gene expression in vivo. In summary, we could demonstrate that IL-2 promoter-driven reporter gene expression in transgenic mice is a sensitive tool to characterize IL-2 expressing cells phenotyplcally. With this new mouse model, It Is hoped to better define the role of IL-2 expression in the activation process of defined anttgenlc responses and in the genesis of experimental or genetic disease models. © 1994 Oxford University Press.
Authors & Co-Authors
Brombacher, Frank
Switzerland, Basel
Preclinical Research
Statistics
Citations: 36
Authors: 1
Affiliations: 1
Identifiers
Doi:
10.1093/intimm/6.2.189
ISSN:
09538178
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study