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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
No Evidence for Selection of HIV-1 with Enhanced Gag-Protease or Nef Function among Breakthrough Infections in the CAPRISA 004 Tenofovir Microbicide Trial
PLoS ONE, Volume 8, No. 8, Article e71758, Year 2013
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Description
Background:Use of antiretroviral-based microbicides for HIV-1 prophylaxis could introduce a transmission barrier that inadvertently facilitates the selection of fitter viral variants among incident infections. To investigate this, we assessed the in vitro function of gag-protease and nef sequences from participants who acquired HIV-1 during the CAPRISA 004 1% tenofovir microbicide gel trial.Methods and Results:We isolated the earliest available gag-protease and nef gene sequences from 83 individuals and examined their in vitro function using recombinant viral replication capacity assays and surface protein downregulation assays, respectively. No major phylogenetic clustering and no significant differences in gag-protease or nef function were observed in participants who received tenofovir gel versus placebo gel prophylaxis.Conclusion:Results indicate that the partial protective effects of 1% tenofovir gel use in the CAPRISA 004 trial were not offset by selection of transmitted/early HIV-1 variants with enhanced Gag-Protease or Nef fitness. © 2013 Chopera et al.
Authors & Co-Authors
Chopera, Denis R.
South Africa, Durban
The Nelson R. Mandela Medical School
South Africa, Johannesburg
National Health Laboratory Service
Canada, Burnaby
Simon Fraser University
Canada, Vancouver
British Columbia Centre for Excellence in Hiv-aids
Mann, Jaclyn Kelly
South Africa, Durban
The Nelson R. Mandela Medical School
Mwimanzi, Philip
Canada, Burnaby
Simon Fraser University
Omarjee, Saleha
South Africa, Durban
The Nelson R. Mandela Medical School
Kuang, Xiaomei T.
Canada, Burnaby
Simon Fraser University
Ndabambi, Nonkululeko
South Africa, Johannesburg
National Health Laboratory Service
Goodier, Sarah A.M.
South Africa, Johannesburg
National Health Laboratory Service
Martin, Eric
Canada, Burnaby
Simon Fraser University
Canada, Vancouver
British Columbia Centre for Excellence in Hiv-aids
Naranbhai, Vivek
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Abdool Karim, Salim S.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Abdool Karim, Quarraisha Abdool
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Brumme, Zabrina L.
Canada, Burnaby
Simon Fraser University
Canada, Vancouver
British Columbia Centre for Excellence in Hiv-aids
Ndung'u, Thumbi P.
South Africa, Durban
The Nelson R. Mandela Medical School
Germany, Berlin
Max Planck Institute for Infection Biology
Williamson, Carolyn
South Africa, Johannesburg
National Health Laboratory Service
Brockman, Mark A.
Canada, Burnaby
Simon Fraser University
Canada, Vancouver
British Columbia Centre for Excellence in Hiv-aids
Statistics
Citations: 15
Authors: 15
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0071758
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Infectious Diseases