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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Production of specific monoclonal antibodies to salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever
Journal of Infectious Diseases, Volume 161, No. 1, Year 1990
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Description
Four murine monoclonal antibodies (MAbs)to Salmonella typhi flagellin were produced. These MAbs did not react with eight other enterobacterial strains tested: Salmonella enteritidis, Salmonella typhimurium, Salmonella paratyphi A, Escherichia coli, Shigella flexneri, Shigella sonnei, Yersinia enterocolitica, and Campylobacter jejuni. Allfour MAbscross-reactedwith Salmonella muenchen flagellin indicating specificityfor d antigenic flagellar epitope. One MAb(C4) was selected to developa double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect S. typhi flagellin in serum samples. By use of this assay S. typhi flagellar antigen was detected in 95.5% of serum samples from patients with positive hemoculture for S. typhi, in 93.6% of samples from patients with positive serodiagnosis of typhoid fever, in 26% of samples collected from patients who were initially hemoculture-positive for S. typhi and who had undergone 78 d of chemotherapy, in 8.5% of samples from healthy persons from an endemic area, and in no samples from healthy persons from a nonendemic area. The presence of high levelsof flagellin antibody titers did not interfere with the antigen detection. The detection of S. typhi flagellar antigen in patient serum may have practical value for rapid diagnosis of typhoid fever. © 1990 by The University of Chicago. All rights reserved.
Authors & Co-Authors
Sadallah, Fatiha
Switzerland, Geneva
Université de Genève
Brighouse, Guy
Switzerland, Geneva
Université de Genève
del Giudice, Giuseppe
Switzerland, Geneva
Université de Genève
Drager-Dayal, Renu
Switzerland, Geneva
Université de Genève
Hodne, Mouloud
Switzerland, Geneva
Université de Genève
Lambert, Paul Henri
Algeria, Algiers
Ehs en Infectiologie el Hadi Flici Ex-el Kettar
Statistics
Citations: 36
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1093/infdis/161.1.59
ISSN:
00221899
e-ISSN:
15376613
Research Areas
Cancer
Health System And Policy
Infectious Diseases