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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains
FEBS Journal, Volume 278, No. 7, Year 2011
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Description
Factor-inhibiting hypoxia-inducible factor (FIH) is an Fe(II)/2- oxoglutarate-dependent dioxygenase that acts as a negative regulator of the hypoxia-inducible factor (HIF) by catalysing β-hydroxylation of an asparaginyl residue in its C-terminal transcriptional activation domain (CAD). In addition to the hypoxia-inducible factor C-terminal transcriptional activation domain (HIF-CAD), FIH also catalyses asparaginyl hydroxylation of many ankyrin repeat domain-containing proteins, revealing a broad sequence selectivity. However, there are few reports on the selectivity of FIH for the hydroxylation of specific residues. Here, we report that histidinyl residues within the ankyrin repeat domain of tankyrase-2 can be hydroxylated by FIH. NMR and crystallographic analyses show that the histidinyl hydroxylation occurs at the β-position. The results further expand the scope of FIH-catalysed hydroxylations. © 2011 The Authors Journal compilation © 2011 FEBS.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3569879/bin/febs0278-1086-sd1.zip
Authors & Co-Authors
Yang, Ming
United Kingdom, Oxford
University of Oxford
Chowdhury, Rasheduzzaman
United Kingdom, Oxford
University of Oxford
Ge, Wei
United Kingdom, Oxford
University of Oxford
Egypt, Asyut
Faculty of Pharmacy
Hamed, Refaat B.
United Kingdom, Oxford
University of Oxford
McDonough, Michael A.
United Kingdom, Oxford
University of Oxford
Claridge, Timothy D.W.
United Kingdom, Oxford
University of Oxford
Kessler, Benedikt M.
United Kingdom, Oxford
Nuffield Department of Medicine
Cockman, Matthew E.
United Kingdom, Oxford
Nuffield Department of Medicine
Ratcliffe, Peter J.
United Kingdom, Oxford
Nuffield Department of Medicine
Schofield, Christopher J.
United Kingdom, Oxford
University of Oxford
Statistics
Citations: 71
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1111/j.1742-4658.2011.08022.x
ISSN:
1742464X
e-ISSN:
17424658