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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Spectrophotometric and fluorimetric determination of diazepam, bromazepam and clonazepam in pharmaceutical and urine samples
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Volume 60, No. 4, Year 2004
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Description
New spectrophotometric and fluorimetric methods have been developed to determine diazepam, bromazepam and clonazepam (1,4-benzodiazepines) in pure forms, pharmaceutical preparations and biological fluid. The new methods are based on measuring absorption or emission spectra in methanolic potassium hydroxide solution. Fluorimetric methods have proved selective with low detection limits, whereas photometric methods showed relatively high detection limits. Successive applications of developed methods for drugs determination in pharmaceutical preparations and urine samples were performed. Photometric methods gave linear calibration graphs in the ranges of 2.85-28.5, 0.316-3.16, and 0.316-3.16μgml-1 with detection limits of 1.27, 0.08 and 0.13μgml-1 for diazepam, bromazepam and clonazepam, respectively. Corresponding average errors of 2.60, 5.26 and 3.93 and relative standard deviations (R.S.D.s) of 2.79, 2.12 and 2.83, respectively, were obtained. Fluorimetric methods gave linear calibration graphs in the ranges of 0.03-0.34, 0.03-0.32 and 0.03-0.38μgml-1 with detection limits of 7.13, 5.67 and 16.47ngml-1 for diazepam, bromazepam and clonazepam, respectively. Corresponding average errors of 0.29, 4.33 and 5.42 and R.S.D.s of 1.27, 1.96 and 1.14 were obtained, respectively. Statistical Students t-test and F-test have been used and satisfactory results were obtained. © 2003 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Salem, Alaa A.
United Arab Emirates, Al Ain
United Arab Emirates University
Egypt, Giza
Cairo University
Barsoum, Barsoum N.
Egypt, Giza
Faculty of Science
Izake, Emad L.
Egypt, Cairo
Forensic Medicine Authority
Statistics
Citations: 70
Authors: 3
Affiliations: 4
Identifiers
Doi:
10.1016/S1386-1425(03)00300-7
ISSN:
13861425
Research Areas
Environmental