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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Filamin a binds to CCR2B and regulates its internalization
PLoS ONE, Volume 5, No. 8, Article e12212, Year 2010
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Description
The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. Employing the yeast two-hybrid system, we identified the actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. Co-immunoprecipitation experiments and in vitro pull down assays demonstrated that FLNa binds constitutively to CCR2B. The colocalization of endogenous CCR2B and filamin A was detected at the surface and in internalized vesicles of THP-1 cells. In addition, CCR2B and FLNa were colocalized in lamellipodia structures of CCR2Bexpressing A7 cells. Expression of the receptor in filamin-deficient M2 cells together with siRNA experiments knocking down FLNa in HEK293 cells, demonstrated that lack of FLNa delays the internalization of the receptor. Furthermore, depletion of FLNa in THP-1 monocytes by RNA interference reduced the migration of cells in response to MCP-1. Therefore, FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures. © 2010 Minsaas et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2923182/bin/pone.0012212.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2923182/bin/pone.0012212.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2923182/bin/pone.0012212.s003.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2923182/bin/pone.0012212.s004.tif
Authors & Co-Authors
Minsaas, Laura
Norway, Bergen
Universitetet I Bergen
Planagumà, Jesús
Norway, Bergen
Universitetet I Bergen
Madziva, Michael Taurai
South Africa, Cape Town
University of Cape Town
Krakstad, Beate F.
Norway, Bergen
Universitetet I Bergen
Masià-Balagué, Miriam
Spain, Barcelona
Csic - Instituto de Biologia Molecular de Barcelona Ibmb
Katz, Arieh Anthony
South Africa, Cape Town
University of Cape Town
Aragay, Anna M.
Norway, Bergen
Universitetet I Bergen
Spain, Barcelona
Csic - Instituto de Biologia Molecular de Barcelona Ibmb
Statistics
Citations: 27
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0012212
e-ISSN:
19326203