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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Fab MAbs specific to HA of influenza virus with H5N1 neutralizing activity selected from immunized chicken phage library
Biochemical and Biophysical Research Communications, Volume 395, No. 4, Year 2010
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Description
Hemagglutinin protein (HA) was considered to be the primary target for monoclonal antibody production. This protein not only plays an important role in viral infections, but can also be used to differentiate H5N1 virus from other influenza A viruses. Hence, for diagnostic and therapeutic applications, it is important to develop anti-HA monoclonal antibody (MAb) with high sensitivity, specificity, stability, and productivity. Nine unique Fab MAbs were generated from chimeric chicken/human Fab phage display library constructed from cDNA derived from chickens immunized with recombinant hemagglutinin protein constructed from H5N1 avian influenza virus (A/Vietnam/1203/04). The obtained Fab MAbs showed several characteristics for further optimization and development-three clones were highly specific to only H5N1 virus. This finding can be applied to the development of H5N1 diagnostic testing. Another clone showed neutralization activity that inhibited H5N1 influenza virus infection in Madin-Darby canine kidney (MDCK) cells. In addition, one clone showed strong reactivity with several of the influenza A virus subtypes tested. The conversion of this clone to whole IgG is a promising study for a cross-neutralization activity test. © 2010 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Pitaksajjakul, Pannamthip
Thailand, Bangkok
Faculty of Tropical Medicine, Mahidol University
Lekcharoensuk, Porntippa
Thailand, Bangkok
Kasetsart University
Upragarin, Narin
Thailand, Bangkok
Kasetsart University
Barbas, Carlos F.
United States, San Diego
Scripps Research Institute
Ibrahim, Madiha Salah
Egypt, Alexandria
Faculty of Veterinary Medicine
Ikuta, Kazuyoshi
Japan, Suita
Research Institute for Microbial Diseases
Ramasoota, Pongrama
Thailand, Bangkok
Faculty of Tropical Medicine, Mahidol University
Statistics
Citations: 29
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.bbrc.2010.04.040
ISSN:
0006291X
e-ISSN:
10902104
Research Areas
Genetics And Genomics