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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Opposing roles for 53BP1 during homologous recombination
Nucleic Acids Research, Volume 41, No. 21, Year 2013
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Description
Although DNA non-homologous end-joining repairs most DNA double-strand breaks (DSBs) in G2 phase, late repairing DSBs undergo resection and repair by homologous recombination (HR). Based on parallels to the situation in G1 cells, previous work has suggested that DSBs that undergo repair by HR predominantly localize to regions of heterochromatin (HC). By using H3K9me3 and H4K20me3 to identify HC regions, we substantiate and extend previous evidence, suggesting that HC-DSBs undergo repair by HR. Next, we examine roles for 53BP1 and BRCA1 in this process. Previous studies have shown that 53BP1 is pro-non-homologous end-joining and anti-HR. Surprisingly, we demonstrate that in G2 phase, 53BP1 is required for HR at HC-DSBs with its role being to promote phosphorylated KAP-1 foci formation. BRCA1, in contrast, is dispensable for pKAP-1 foci formation but relieves the barrier caused by 53BP1. As 53BP1 is retained at irradiation-induced foci during HR, we propose that BRCA1 promotes displacement but retention of 53BP1 to allow resection and any necessary HC modifications to complete HR. In contrast to this role for 53BP1 in HR in G2 phase, we show that it is dispensable for HR in S phase, where HC regions are likely relaxed during replication. © The Author(s) 2013. Published by Oxford University Press.
Authors & Co-Authors
Kakarougkas, Andreas
United Kingdom, Brighton
University of Sussex
Ismail, A. M.
United Kingdom, Brighton
University of Sussex
Shibata, Atsushi K.
United Kingdom, Brighton
University of Sussex
Löbrich, Markus
Germany, Darmstadt
Technische Universität Darmstadt
Jeggo, Penny A.
United Kingdom, Brighton
University of Sussex
Statistics
Citations: 67
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1093/nar/gkt729
ISSN:
13624962
Research Areas
Genetics And Genomics