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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Reusable impedimetric aptasensor
Analytical Chemistry, Volume 77, No. 19, Year 2005
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Description
A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN) 63-/4-, using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by electrochemical impedance spectroscopy or cyclic voltammetry. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution, and the immobilized aptamer subsequently was used for repeated detection of thrombin. The aptamer-functionalized electrode showed a linear response of the charge-transfer resistance to the increase of thrombin concentration in the range of 5.0-35.0 nM and the thrombin was easily detectable to a concentration of 2.0 nM. © 2005 American Chemical Society.
Authors & Co-Authors
Radi, Abd Elgawad
Egypt, New Damietta
Faculty of Science
Acero Sánchez, Josep Lluís
Spain, Tarragona
Universitat Rovira I Virgili
Baldrich, Eva
Spain, Tarragona
Universitat Rovira I Virgili
O’ Sullivan, Ciara K.
Spain, Tarragona
Universitat Rovira I Virgili
Spain, Barcelona
Institució Catalana de Recerca I Estudis Avançats
Statistics
Citations: 240
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1021/ac0505775