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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Screening of novel malaria DNA vaccine candidates using full-length cDNA library
Experimental Parasitology, Volume 135, No. 3, Year 2013
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Description
No licensed malaria vaccine exists, in spite of intensive development efforts. We have been investigating development of a DNA vaccine to prevent malaria infection. To date, we have established a full-length cDNA expression library from the erythrocytic-stage murine malaria parasite, Plasmodium berghei. We found that immunization of mice with combined 2000 clones significantly prolonged survival after challenge infection and that splenocytes from the immunized mice showed parasite-specific cytokine production. We determined the 5'-end one-pass sequence of these clones and mapped a draft genomic sequence for P. berghei for use in screening vaccine candidates for efficacy.In this study, we annotated these cDNA clones by comparing them with the genomic sequence of Plasmodium falciparum. We then divided them into several subsets based on their characteristics and examined their protective effects against malaria infection. Consequently, we selected 104 clones that strongly induced specific IgG production and decreased the mortality rate in the early phase.Most of these 104 clones coded for unknown proteins. The results suggest that these clones represent potential novel malaria vaccine candidates. © 2013 Elsevier Inc.
Authors & Co-Authors
Shibui, Akiko
Japan, Tokyo
The University of Tokyo
Japan, Tokyo
Tokyo University of Science
Nakae, Susumu
Japan, Tokyo
The University of Tokyo
Japan, Saitama
Science and Technology Agency
Watanabe, Junichi
Japan, Tokyo
The University of Tokyo
Sato, Yoshitaka
Japan, Tokyo
Tokyo University of Science
Tolba, Mohammed E.M.
Japan, Tokyo
The University of Tokyo
Egypt, Asyut
Assiut University
Doi, Junko
Japan, Tokyo
Nihon University
Shiibashi, Takashi
Japan, Tokyo
Nihon University
Nogami, Sadao
Japan, Tokyo
Nihon University
Sugano, Sumino
Japan, Tokyo
The University of Tokyo
Hozumi, Nobumichi
Japan, Tokyo
Tokyo University of Science
Japan, Urayasu
Ryotokuji University
Statistics
Citations: 10
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1016/j.exppara.2013.09.007
ISSN:
00144894
e-ISSN:
10902449
Research Areas
Genetics And Genomics
Infectious Diseases
Maternal And Child Health