In vitro killing of mycobacteria by low temperature atmospheric pressure plasma and dielectric barrier discharge plasma for treatment of tuberculosis

Background To prove the efficacy of cold atmospheric plasma (CAP) to inactivate mycobacteria in vitro, we tested the susceptibility of important mycobacterial species to evaluate potential alternative treatment options. Methods We tested 8 different mycobacterial species M. tuberculosis2 (MTB) (resistant against isoniazid (INH)), M. marinum, M. ulcerans, M. abscessus, M. chelonae, M. africanum, M. terrae and M. avium against two typical CAP devices, a low temperature atmospheric pressure plasma jet (APPJ) and a dielectric barrier discharge plasma (DBD). The tests followed a modified standard procedure by comparing obtained inhibition zones after irradiation of 3, 15, 30 and 60 s on selective (Löwenstein-Jensen) medium. Results Irradiation by both sources proved high antimycobacterial killing effectiveness against all species including the INH-resistant strain of M. tuberculosis and a set of most important mycobacterial pathogens causing mycobacteriosis. Plasma irradiation