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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Decreased in vitro susceptibility of plasmodium falciparum isolates to artesunate, mefloquine, chloroquine, and quinine in Cambodia from 2001 to 2007
Antimicrobial Agents and Chemotherapy, Volume 54, No. 5, Year 2010
Notification
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Description
This study describes the results of in vitro antimalarial susceptibility assays and molecular polymorphisms of Plasmodium falciparum isolates from Cambodia. The samples were collected from patients enrolled in therapeutic efficacy studies (TES) conducted by the Cambodian National Malaria Control Program for the routine efficacy monitoring of artemisinin-based combination therapy (ACT) (artesunate-mefloquine and artemether-lumefantrine combinations). The isolates (n=2,041) were obtained from nine sentinel sites during the years 2001 to 2007. Among these, 1,588 were examined for their in vitro susceptibilities to four antimalarials (artesunate, mefloquine, chloroquine, and quinine), and 851 isolates were genotyped for single nucleotide polymorphisms (SNPs). The geometric means of the 50% inhibitory concentrations (GMIC 50s) of the four drugs tested were significantly higher for isolates from western Cambodia than for those from eastern Cambodia. GMIC50s for isolates from participants who failed artesunate-mefloquine therapy were significantly higher than those for patients who were cured (P, <0.001). In vitro correlation of artesunate with the other drugs was observed. The distributions of the SNPs differed between eastern and western Cambodia, suggesting different genetic backgrounds of the parasite populations in these two parts of the country. The GMIC50s of the four drugs tested increased significantly in eastern Cambodia during 2006 to 2007. These results are worrisome, because they may signal deterioration of the efficacy of artesunate-mefloquine beyond the Cambodian-Thai border. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Lim, Pharath
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Wongsrichanalai, Chansuda
Indonesia, Jakarta
U.s. Naval Medical Research Unit No. 2, Jakarta
Chim, Pheaktra
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Khim, Nimol
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Kim, Saorin
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Chy, Sophy
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Sem, Rithy
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Nhem, Sina
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Yi, Poravuth
Unknown Affiliation
Socheat, Duong
Unknown Affiliation
Genton, Blaise
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Beck, Hans Peter
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Rogers, William Oscar
Indonesia, Jakarta
U.s. Naval Medical Research Unit No. 2, Jakarta
Coppeé, Jean Yves
France, Evry
Genopole
Fandeur, Thierry
France, Paris
Institut Pasteur, Paris
Mercereau-Puijalon, Odile
France, Paris
Institut Pasteur, Paris
Ringwald, Pascal
Switzerland, Geneva
Organisation Mondiale de la Santé
Le Bras, Jacques L.
Switzerland, Geneva
Organisation Mondiale de la Santé
Ariey, Frédéric
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Statistics
Citations: 72
Authors: 19
Affiliations: 7
Identifiers
Doi:
10.1128/AAC.01304-09
ISSN:
00664804
Research Areas
Genetics And Genomics
Infectious Diseases