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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Reconstitution of cytomegalovirus specific T cells after pediatric allogeneic stem cell transplantation: Results from a pilot study using a multi-allele CMV tetramer group
Klinische Padiatrie, Volume 220, No. 6, Year 2008
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Description
Background: Recovery of cytomegalovirus (CMV)-specific T cell mediated immunity after allogeneic hematopoietic stem cell transplantation (SCT) is critical for protection against CMV disease. Tetramer-based technologies have been shown to be a sensitive tool in the enumeration of specific T cells, but have the disadvantage of HLA-restriction of the peptides. Patients and Methods: In this pilot study, we tested the feasibility of a panel of 6 CMV-specific tetrameric HLA/CMV-peptide complexes to enumerate CMV-specific CD8+T cells (CTLs). The reconstitution of CMV-specific CTLs was assessed in 16 children in the first year after allogeneic SCT (median age, 8 years). Results: The presented assay covered more than 85% of our patients transplanted in the last 3 years. During CMV-reactivation, all 4 of the 16 analyzed patients with a high virus-load showed less than 10 CMV-specific CTLs/μI; out of these, three had not any detectable CMV-CTLs. On the other hand, five of the children with less than 10 CMV-specific CTLs/μI did not develop CMV reactivation. When enumeration of T cells was performed by means of different tetrameric HLA/ CMV-peptide complexes simultaneously, the numbers of CMV-specific CTLs cells widely differed according to the HLA-type. Conclusions: Our pilot study suggests that enumeration of CMV-specific T cells by means of a panel of 6 tetramers might be a useful tool in the risk assessment for CMV reactivation in the majority of patients undergoing allogeneic SCT, but future trials have to evaluate whether this method is appropriate in tailoring antiviral therapy in the individual patient. © Georg Thieme Verlag KG Stuttgart.
Authors & Co-Authors
Koehl, Ulrike
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Germany, Frankfurt am Main
Goethe-universität Frankfurt am Main
Dirkwinkel, E.
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Koenig, M.
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Erben, S.
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Soerensen, J.
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Bader, Peter
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Doérr, Hans Wilhelm
Germany, Frankfurt am Main
Institute of Medical Virology
Preiser, Wolfgang
South Africa, Stellenbosch
Stellenbosch University
Weissinger, E.
Germany, Hannover
Hannover Medical School
Klingebiel, Thomas Erich
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Martin, Hans
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Lehrnbecher, Thomas
Germany, Frankfurt am Main
Universitätsklinikum Frankfurt
Statistics
Citations: 14
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1055/s-0028-1086029
Research Areas
Genetics And Genomics
Health System And Policy
Maternal And Child Health