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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Erythrocyte invasion and merozoite ligand gene expression in severe and mild plasmodium falciparum malaria
Journal of Infectious Diseases, Volume 201, No. 3, Year 2010
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Description
Erythrocyte invasion is central to malaria parasite replication and virulence. Plasmodium falciparum parasites use different alternative erythrocyte receptors and vary in expression of erythrocyte-binding antigenic (EBA) proteins and reticulocyte-binding protein homologues (Rh). Parasite invasion phenotypes and schizont-stage transcript expression profiles of the 8 eba and Rh protein-coding genes without internal stop codons were determined for 163 clinical isolates cultured ex vivo in The Gambia. There was extensive diversity in ability to invade erythrocytes treated with neuraminidase, trypsin, or chymotrypsin, and severe malaria isolates were less restricted by trypsin treatment than were mild malaria isolates (P = .015). Expression profiles of the eba and Rh genes showed distinct clusters indicating coordinated alternative transcription. The most divergent of 5 major clusters was dominated by Rh2b, with virtually no expression of eba175 or eba140 genes (which were dominant in the other 4 clusters). Particular transcripts were significantly correlated with parasitemia (Rh5 was positively correlated and eba140 negatively correlated; P < .001 for both) and age of patients (eba181 was positively correlated and eba175 negatively correlated; P< .01 for both) but not with invasion phenotypes or severity of malaria. Severe and mild malaria isolates were also evenly represented across the different expression clusters. © 2010 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Gomez-Escobar, Natalia
Gambia, Banjul
Medical Research Council Laboratories Gambia
Amambua-Ngwa, Alfred
Gambia, Banjul
Medical Research Council Laboratories Gambia
Walther, Michael
Gambia, Banjul
Medical Research Council Laboratories Gambia
Okebe, Joseph U.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Ebonyi, Augustine Odo
Gambia, Banjul
Medical Research Council Laboratories Gambia
Conway, David J.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Statistics
Citations: 77
Authors: 6
Affiliations: 1
Identifiers
Doi:
10.1086/649902
ISSN:
00221899
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Gambia