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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
veterinary
Detection of salamonella gallinarum and S. Typhimurium DNA in experimentally infected chicks by polymerase chain reaction
Journal of Veterinary Medical Science, Volume 57, No. 1, Year 1995
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Description
DNA detection with polymerase chain reaction (PCR) as a mean of identifying Salmonella infection in chickens was compared with the conventional culture procedure. DNA was extracted from organs of experimentally infected chicks with either S. Gallinarum or S. Typhimurium. The pair of primers used were those directed at the InvA gene. Bacteria isolation was done by inoculating the pre-enrichment media with samples. As was expected a 284 bp fragment DNA was amplified from extracted DNA of infected organs by PCR. The results of our studies indicate that the PCR method is more sensitive than the conventional culture procedure since we were able to detect both S. Gallinarum and S. Typhimurium DNA not only in samples positive for bacteria isolation but also in negative samples. It was possible to detect Salmonella DNA in 15 out of 20 organ samples from chicks infected with S. Gallinarum 21 hr after infection, but, only five were positive for bacteria isolation. Salmonella DNA was detected throughout the entire test period. The results of this study confirm that PCR is a useful tool for the detection of Salmonella infection in poultry. © 1995, JAPANESE SOCIETY OF VETERINARY SCIENCE. All rights reserved.
Authors & Co-Authors
Tuchili, Lawrence Musonda
Zambia, Lusaka
Zambia University
Kodama, Hiroshi
Japan, Osaka
Osaka Metropolitan University
Izumoto, Yoko
Japan, Osaka
Osaka Metropolitan University
Mukamoto, Masafumi
Japan, Osaka
Osaka Metropolitan University
Baba, Tsuyoshi
Japan, Osaka
Osaka Metropolitan University
Fukata, Tsuneo
Japan, Osaka
Osaka Metropolitan University
Statistics
Citations: 18
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1292/jvms.57.59
e-ISSN:
13477439
Research Areas
Genetics And Genomics
Infectious Diseases